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Bburgdor25kdabcsfqlib

Published: August 9, 2026Updated: August 16, 2026

This test detects antibodies against the 25kD protein of Borrelia burgdorferi (Lyme disease bacteria) in cerebrospinal fluid to help diagnose nervous system involvement.

Quick summary

{"The test looks for specific antibodies in the fluid surrounding the brain and spinal cord","It helps diagnose neuroborreliosis (Lyme disease affecting the nervous system)","A positive result suggests the bacteria may have spread to the central nervous system","The test is performed using immunoblot (Western blot) technology","Results are typically reported as positive or negative for the 25kD antibody"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
12864-5
Short code
B25CSFBLOT
Synonyms
B burgdor25kD Ab CSF Ql IB
Category
chemistry
Sample
csf
Container
Sterile, leak-proof, screw-cap CSF collection tube (polypropylene), preferably cryovial
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
Immunoblot (Western Blot)
CPT
86618
SNOMED
122388004
Specimen volume
0.5-1 mL cerebrospinal fluid
Stability
Room temperature: 2 hours; Refrigerated (2-8°C): 24 hours; Frozen (-20°C or below): up to 6 months; avoid repeated freeze-thaw cycles
Hemolysis max
200
TAT
48–96 hours
Order
routine

What is this test?

This test detects antibodies specifically targeting the 25kD protein of Borrelia burgdorferi, the bacterium that causes Lyme disease. The test is performed on cerebrospinal fluid (CSF), the clear fluid that surrounds and protects the brain and spinal cord. Using a technique called immunoblot (or Western blot), laboratory specialists can identify whether your immune system has produced specific antibodies against this bacterial protein.

Why is it ordered?

This test is primarily ordered when a patient shows symptoms of Lyme disease affecting the nervous system (neuroborreliosis). Symptoms may include facial palsy, meningitis, nerve pain, numbness, or tingling in the limbs. It helps doctors determine if the Lyme disease bacteria have spread to the central nervous system, which is important for guiding treatment decisions.

How is the sample collected?

The sample is cerebrospinal fluid, which is collected through a procedure called a lumbar puncture (spinal tap). During this procedure, a healthcare provider inserts a thin needle between the bones in the lower spine to withdraw a small amount of CSF. The procedure is generally safe but may cause temporary back pain or headache.

What do the results mean?

Results are typically reported as 'Positive' or 'Negative' for the 25kD antibody. A positive result indicates that antibodies against the Borrelia burgdorferi 25kD protein were detected in your CSF, suggesting the bacteria may have spread to your central nervous system. A negative result means no antibodies were detected, though this does not completely rule out Lyme disease—early infection or treatment with antibiotics may affect results.

Reference range

Negative (No Borrelia burgdorferi 25kD antibodies detected)N/A

Specimen: csf

No special patient preparation required. Inform clinician of any current antibiotic therapy as it may affect antibody detection.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is a lumbar puncture painful?
The procedure involves some discomfort. Local anesthesia is used to numb the area, and you may feel pressure or a brief sting during the needle insertion. Most people experience only mild to moderate back soreness afterward, which usually resolves within a few days.
How long does it take to get results?
Immunoblot testing is more complex than routine blood tests and typically takes several days to a week to complete. The laboratory must first detect antibodies and then confirm them using the immunoblot technique against specific bacterial proteins.
Can this test give false results?
Like all tests, there is a possibility of false results. False positives can occur due to cross-reactions with other infections, while false negatives can happen if the test is performed too early in the infection before antibodies have developed in the CSF, or if the patient has already received antibiotics.
Do I need to fast before this test?
No fasting is required for this test. However, you should inform your doctor about any medications or supplements you are taking, as some may affect test results.
  1. 1
    CDC - Lyme Disease Diagnosis and TestingSource
  2. 2
    Mayo Clinic - Lyme DiseaseSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy