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Understanding Your Laboratory Test: A Patient Guide to What the Test Is, How the Sample Is Collected, Why It Is Done, and What the Results Mean

Published: August 9, 2026Updated: August 17, 2026

This test detects benzodiazepine medications in stomach fluid to help diagnose poisoning or overdose.

Quick summary

{"Measures benzodiazepine levels in stomach fluid","Helps diagnose accidental or intentional overdose","Sample collected through stomach tube or aspiration","Results help doctors determine treatment","Different benzodiazepines may show different concentrations"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
33560-4
Short code
BENZGAS
Synonyms
Benzodiaz Gast-mCnc
Category
chemistry
Sample
serum
Container
Sterile, leak-proof plastic container without preservative; gastight container with sodium fluoride may be used for preservation
Units
N/A
Ref low
0
Ref high
50
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
Immunoassay with confirmation by Gas Chromatography-Mass Spectrometry (GC-MS) or Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT
80307
SNOMED
395171001
Specimen volume
5-10 mL of gastric fluid
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–48 hours
Order
routine

This test is a laboratory analysis that measures specific substances in your body to help your doctor evaluate your overall health, screen for certain conditions, diagnose a possible illness, monitor an ongoing disease, or check how well a treatment is working over time.

The Benzodiazepine test in gastric fluid is a laboratory test that detects and measures the presence of benzodiazepine medications in the stomach contents. Benzodiazepines are a class of prescription drugs commonly used to treat anxiety, insomnia, seizures, and muscle spasms. Examples include diazepam (Valium), lorazepam (Ativan), alprazolam (Xanax), and clonazepam (Klonopin).

A trained healthcare professional will collect your sample using a standard procedure, which may involve drawing a small amount of blood from a vein in your arm, obtaining a urine specimen in a sterile container, or taking a swab from the affected area, and the process is usually quick and well tolerated.

The sample is collected from the stomach through a nasogastric (NG) tube or during gastric lavage (stomach pumping). This procedure is typically performed in an emergency department or hospital setting when a benzodiazepine overdose is suspected. The healthcare provider may also collect vomit or stomach contents if the patient has vomited.

Your doctor orders this test to gather objective information about what is happening inside your body, confirm or rule out a suspected condition, establish a baseline before starting a new medication, track changes in your health between visits, or adjust your treatment plan based on measurable findings.

This test is primarily ordered when a patient shows symptoms of drug poisoning or overdose, such as extreme drowsiness, confusion, slurred speech, difficulty breathing, or loss of consciousness. It helps healthcare providers determine if benzodiazepines are involved and guide appropriate treatment. The test may also be used in forensic cases or when monitoring patients for drug exposure.

Your results will show whether your values fall within the normal reference range for this test; results within range generally suggest no abnormality, while values that are higher or lower than expected may point to a condition that needs further evaluation, and your doctor will interpret them together with your symptoms, medical history, and other test findings before recommending next steps.

A positive result indicates that benzodiazepines are present in the stomach. The concentration may help estimate how much was ingested and how long ago. However, the amount in stomach fluid does not directly correlate with blood levels or toxicity. A negative result does not completely rule out benzodiazepine use, as the drugs may have already moved out of the stomach or been metabolized.

Reference range

Negative: <50 ng/mL (cutoff varies by laboratory and assay). Therapeutic monitoring ranges are not well established for gastric fluid specimens.N/A

Specimen: serum

No fasting required. Specimen is typically collected via gastric lavage or nasogastric tube in emergency or toxicology settings.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is crucial in emergency medicine for diagnosing benzodiazepine overdose, which can be life-threatening especially when combined with alcohol or other depressants. It helps guide treatment decisions including whether to use the reversal agent flumazenil, provide supportive care, or perform gastric decontamination. The test is also valuable in toxicology screening and forensic investigations where drug ingestion is suspected.

Frequently asked questions

Is this test painful?
The procedure to collect gastric fluid may cause some discomfort. A thin tube is passed through the nose or mouth into the stomach. This may cause gagging or mild throat irritation. The procedure is typically brief and necessary for accurate diagnosis in overdose situations.
How long does it take to get results?
Results are typically available within a few hours to 24 hours, depending on the laboratory. In emergency situations, preliminary results may be available more quickly to guide immediate treatment.
Can this test detect all types of benzodiazepines?
Most standard tests can detect common benzodiazepines. However, some newer or less common formulations may not be detected by all testing methods. If a specific benzodiazepine is suspected, additional specialized testing may be required.
Do I need to fast before this test?
Fasting is not typically required for this test since the sample is collected from the stomach. However, if the test is being done emergently after a suspected overdose, food contents in the stomach may affect the test interpretation.
What happens if the test is positive?
If benzodiazepines are detected, your healthcare team will determine the appropriate treatment based on your symptoms and overall condition. Treatment may include monitoring, supportive care, activated charcoal (if early enough), or specific medications to reverse the effects.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy