Understanding Your Laboratory Test: Why It Is Done, How It Is Performed, and What Your Results Mean
This test checks for the presence of benzodiazepine drugs in your blood to help monitor medication use or investigate suspected overdose.
{"Detects benzodiazepine medications like Valium, Xanax, or Ativan in blood","Used to monitor proper medication use or check for overdose","A blood sample is collected from a vein in your arm","Results are typically reported as positive or negative","Results may be used for medical, legal, or employment purposes"}
Key facts at a glance
- LOINC
- 3389-4
- Short code
- BENZSP
- Synonyms
- Benzodiaz SerPl Ql
- Category
- chemistry
- Sample
- serum
- Container
- Red-top serum collection tube (no anticoagulant) or Tiger-top (SST) serum separator tube; plasma may be collected in lavender-top (EDTA) or green-top (heparin) tubes.
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- Immunoassay (qualitative screen); positive results typically confirmed by Gas Chromatography-Mass Spectrometry (GC-MS) or Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
- CPT
- 80307
- SNOMED
- 33361009
- Specimen volume
- 5-10 mL of whole blood yielding approximately 2-4 mL of serum or plasma
- Stability
- Serum/plasma stable at room temperature (20-25°C) for 24 hours, refrigerated (2-8°C) for 7 days, and frozen (-20°C or below) for 30 days. Avoid repeated freeze-thaw cycles.
- Hemolysis max
- 200
- Lipemia max
- 500
- Icterus max
- 25
- TAT
- 4–72 hours
- Order
- routine
This test is a laboratory procedure that helps your doctor check your overall health, screen for certain medical conditions, or monitor a condition you already have by analyzing a small sample in a controlled setting.
The benzodiazepines blood test detects the presence of benzodiazepine medications in your serum or plasma. Benzodiazepines are a class of prescription drugs commonly used to treat anxiety, insomnia, seizures, muscle spasms, and as sedatives before medical procedures. Common examples include diazepam (Valium), alprazolam (Xanax), lorazepam (Ativan), clonazepam (Klonopin), and temazepam (Restoril).
A trained healthcare professional will collect your sample at a clinic, hospital, or laboratory, following careful steps to keep you comfortable and to make sure the specimen is handled correctly for accurate results.
A healthcare professional will draw a small blood sample from a vein in your arm using a needle. The blood is collected into a special tube and sent to a laboratory for analysis. The test looks for traces of benzodiazepine drugs or their breakdown products (metabolites) in your blood. You do not need to fast before this test, but you should inform your doctor about all medications, supplements, or herbal products you are currently taking.
Your results are compared against standard reference ranges, and your doctor will explain what the values indicate about your health, whether a treatment is working, or whether additional testing may be needed.
Results are typically reported as either 'Positive' (benzodiazepines detected) or 'Negative' (no benzodiazepines detected). A positive result means benzodiazepine drugs were found in your blood. The specific level detected may not indicate exactly how much you took, as levels vary based on the type of drug, dosage, time since ingestion, and individual metabolism. Your healthcare provider will interpret the results in the context of your medical history and symptoms.
Most people experience only minor, temporary effects, such as brief discomfort or slight bruising where the sample was collected, and serious complications from this test are very rare.
The risks associated with this test are minimal and similar to routine blood draws. You may experience slight pain or bruising at the injection site, dizziness, or rarely, infection. These side effects are usually temporary and resolve on their own.
Reference range
Negative (Cutoff: 200 ng/mL for most benzodiazepines; varies by specific compound and laboratory protocol)N/A
No fasting required. Patient should be informed that certain prescription and over-the-counter medications may cross-react with the assay. Specimen collection should ideally occur prior to the next scheduled dose for therapeutic drug monitoring purposes.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Monitoring medication compliance: Ensures patients are taking their prescribed benzodiazepine medication as directed by their healthcare provider.
Diagnosing overdose: Helps identify benzodiazepine overdose in patients presenting with symptoms such as excessive drowsiness, slurred speech, confusion, impaired coordination, or slowed breathing.
Evaluating unexplained symptoms: Assists in determining whether benzodiazepines may be contributing to unusual symptoms or drug interactions.
Forensic and legal purposes: May be used in legal investigations, workplace drug testing programs, or substance abuse monitoring as part of a comprehensive drug screening panel.
Frequently asked questions
How long do benzodiazepines stay in your blood?
Do I need to stop my medication before the test?
Will a positive result show exactly which benzodiazepine I took?
Can the test give false results?
How long does it take to get results?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy