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Serum Hemoglobin A1c (HbA1c) with Electrophoresis-Based Measurement

Published: August 9, 2026Updated: August 15, 2026

This test measures the percentage of hemoglobin that is coated with sugar, reflecting your average blood sugar levels over the past 2–3 months.

Quick summary

["Helps doctors track diabetes management and long-term blood sugar control."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 15, 2026Editorial policy

Key facts at a glance

LOINC
109200-6
Short code
BORHMG
Synonyms
B hermsii IgG Msmt Ser
Category
chemistry
Sample
serum
Container
Serum separator tube (SST) or red-top tube
Units
N/A
Ref low
0
Ref high
1
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
Enzyme Immunoassay (EIA) or Immunofluorescence Assay (IFA)
CPT
86619
SNOMED
122593008
Specimen volume
1–2 mL serum (minimum 0.5 mL)
Stability
Room temperature: 8 hours; Refrigerated (2–8°C): 7 days; Frozen (-20°C or below): 30 days. Avoid repeated freeze-thaw cycles.
Hemolysis max
100
Lipemia max
100
Icterus max
50
TAT
24–72 hours
Order
routine

Why This Test Matters in Diabetes Care

HbA1c is a key indicator of how well your diabetes is controlled over time. Unlike daily blood sugar checks, it provides a broader picture of your glucose trends, helping prevent complications like nerve damage or kidney disease.

How the Test Works

Your blood sample is processed to separate hemoglobin types using electrophoresis. The test then quantifies the proportion of hemoglobin linked to sugar, which correlates with your average blood sugar levels.

What Normal Results Mean

While reference ranges vary by lab, lower-than-average HbA1c levels suggest better glucose control, while higher levels may indicate poorer management. Always discuss results with your healthcare provider.

What Abnormal Results Could Indicate

Elevated HbA1c often signals uncontrolled diabetes, while very low levels may reflect recent changes in diet, medication, or illness. Your provider will interpret results in context with your medical history.

Reference range

Negative: <1:64; Equivocal: 1:64–1:128; Positive: ≥1:256. Results should be interpreted in conjunction with clinical findings and exposure history.N/A

Specimen: serum

No fasting required. Patient should report any recent antibiotic use, travel history to endemic areas (e.g., western United States), and known tick exposure. Acute and convalescent samples (2–4 weeks apart) are recommended for seroconversion confirmation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HbA1c (Serum)

Frequently asked questions

How often should I expect this test?
Typically recommended every 3–6 months for people with diabetes to track progress with treatment plans. Your doctor may adjust frequency based on your needs.
Can diet or exercise affect HbA1c results?
Yes, lifestyle changes can influence results. Recent dietary shifts, stress, or illness may temporarily alter levels, so results should be interpreted with your overall health picture in mind.
What if my HbA1c is outside the typical range?
Your provider will discuss next steps, which may include adjusting medications, reviewing diet/exercise plans, or retesting. Never interpret results alone—always consult your healthcare team.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy