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Bifidobacterium species quantitative culture from stool

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence and quantity of beneficial bacteria called bifidobacteria in your stool. Bifidobacteria are often found in healthy gut microbiomes and may support digestion and immune function.

Quick summary

["This test helps assess gut health and identify if beneficial bacteria levels are normal or if they may need support."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
20696-1
Short code
BIFIDOMILK
Synonyms
Bifidobacterium # Mlk Cult
Category
chemistry
Sample
serum
Container
Sterile, leak-proof collection container; sterile screw-cap tube or culturette for transport
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
0–99 years
Method
organism-specific-culture
CPT
87081
SNOMED
442341005
Specimen volume
Minimum 1-5 mL of milk sample
Stability
Refrigerate at 2-8°C and process within 24 hours of collection. For delayed processing, freeze at -70°C or below. Avoid repeated freeze-thaw cycles. Sample should not be stored at room temperature for extended periods as Bifidobacterium viability decreases.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
72–168 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like persistent diarrhea, bloating, or digestive issues that could be linked to an imbalance in gut bacteria. It might also be used to monitor treatment responses for conditions like antibiotic-associated diarrhea or gut microbiome-related disorders.

What to Expect Before the Test

No special preparation is typically needed, but you may be asked to collect a fresh stool sample in a sterile container. Avoid using toilet paper or cleaning agents that could alter the sample. Follow your provider’s instructions carefully.

How the Test Works

A small amount of your stool sample is cultured in a laboratory setting to grow and count bifidobacteria. The test measures their presence and quantity, which can be compared to expected levels in a healthy gut microbiome.

What the Results Mean

Results are qualitative (present or absent) or quantitative (count of bifidobacteria per gram of stool), depending on the method. Low levels may suggest an imbalance in gut bacteria, while normal or high levels are generally considered healthy. Your provider will interpret results in the context of your symptoms and medical history.

Reference range

No established reference range; results reported as organisms per volume (e.g., CFU/mL or organisms/mL). Interpret in clinical context. Bifidobacterium levels vary based on maternal diet, infant feeding practices, and probiotic supplementation.N/A

Specimen: serum

No special patient preparation required. Sample should represent the milk being analyzed (breast milk, cow's milk, or formula). Avoid contamination during collection. For breast milk samples, express directly into sterile container; for commercial milk products, use aseptic technique.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Stool Bifidobacterium Culture

Frequently asked questions

What does a low or high bifidobacterium count mean?
A low count of bifidobacteria may indicate an imbalance in gut bacteria, sometimes linked to conditions like antibiotic use, inflammatory bowel disease, or other gastrointestinal issues. High levels might be normal in some cases but could warrant further evaluation if symptoms persist.
How long does it take to get results?
Results typically take 3 to 7 business days, depending on the laboratory’s processing time. Your provider will contact you once the results are ready.
Can diet or supplements affect this test?
Yes, certain foods, probiotics, or medications (like antibiotics) can influence bifidobacteria levels. Avoid taking probiotics or changing your diet significantly before the test unless instructed by your provider.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy