Skip to content

CD45-Negative Blast Cells, Bone Marrow — Flow Cytometry

Published: August 9, 2026Updated: August 17, 2026

A bone marrow flow cytometry test that identifies and counts blast cells lacking the CD45 marker, often as part of evaluating possible leukemia.

Quick summary

["This test helps detect and characterize abnormal immature blood cells in bone marrow."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
107077-0
Short code
Blasts.CD45 NFr Mar FC
Synonyms
Blasts.CD45 NFr Mar FC
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
10
Ref high
40
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
flow-cytometry
CPT
84484
SNOMED
302774002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This is a flow cytometry test performed on bone marrow to detect and count blast cells that do not express the CD45 protein. Flow cytometry uses fluorescent-tagged antibodies to examine the characteristics of individual cells.

Why might I need this test?

It may be ordered when a blood count, bone marrow examination, or other findings suggest a blood disorder such as leukemia. The results can help classify abnormal cells and guide further testing and treatment.

How is the test performed?

A small bone marrow sample is collected by a clinician and analyzed in the laboratory using flow cytometry. The laboratory determines whether CD45-negative blast cells are present and measures other cell markers that may identify their likely cell lineage.

How do I prepare for the test?

Preparation depends on the bone marrow sampling method and the instructions provided by your care team. You may need blood tests beforehand, and local anesthetic or sedation is sometimes used. Ask your clinician whether you should take any medicines differently and whether you need transportation assistance afterward.

Reference range

Adult: 10-40 U/LN/A

Specimen: serum

No special preparation required. Avoid fatty meals before the test if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CD45-negative blast analysis

Frequently asked questions

Does a positive result mean I have leukemia?
Not necessarily. CD45-negative blast cells may suggest a blood disorder, including leukemia, but other laboratory and clinical findings are needed to confirm the diagnosis.
Does the test require general anesthesia?
Not always. A bone marrow sample may be taken with local anesthetic, with or without sedation, depending on the procedure and the clinical situation.
Can I use my results to decide on treatment?
Treatment decisions should be made with your hematologist because the flow cytometry result is interpreted alongside blood counts, imaging, genetic testing, and your overall health.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy