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Breath Hydrogen (H₂) and Glucose (H₂O₂) Concentration (Exhaled Breath)

Published: August 9, 2026Updated: August 16, 2026

This test measures the levels of hydrogen gas (H₂) and hydrogen peroxide (a glucose-related byproduct) in your exhaled breath. It may help assess how well your body is processing carbohydrates and detecting certain metabolic or digestive conditions.

Quick summary

["This breath test can help identify issues with sugar metabolism or gastrointestinal function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
16997-9
Short code
Breath H2 p Glc
Synonyms
Breath H2 p Glc
Category
glucose
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test detects two key substances in your breath: hydrogen (H₂), which is produced when certain bacteria in your gut break down undigested carbohydrates, and hydrogen peroxide (H₂O₂), a marker related to glucose metabolism. These measurements help evaluate how efficiently your body absorbs and processes food.

Why It’s Done

Your healthcare provider may order this test if you have symptoms like bloating, gas, diarrhea, or unexplained weight changes that suggest digestive issues or carbohydrate malabsorption. It can also be used in research or specialized clinical settings to study metabolic processes.

How the Test Works

You’ll breathe into a special device that collects your exhaled air. The device analyzes the gases for hydrogen and hydrogen peroxide levels. The results are compared to a baseline to identify any abnormalities in carbohydrate digestion or metabolism.

Preparation Tips

Follow your provider’s instructions carefully. Avoid eating or drinking anything for at least 2 hours before the test, and refrain from smoking or chewing gum to prevent interference. Wear loose clothing for comfort.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Breath H₂ and Glucose Test

Frequently asked questions

What does elevated or abnormal hydrogen or hydrogen peroxide mean?
Higher-than-normal hydrogen levels may indicate issues like lactose intolerance, small intestinal bacterial overgrowth (SIBO), or other digestive disorders where undigested food ferments in your gut. Elevated hydrogen peroxide could suggest metabolic changes or carbohydrate malabsorption.
What does this test help diagnose?
This test is primarily exploratory or research-focused and may not be standard for diagnosing conditions like diabetes or celiac disease. It can, however, support further investigation into gastrointestinal or metabolic concerns when other tests are inconclusive.
How long will results take?
Results from this test are typically processed quickly, often within a few hours or the same day, depending on lab capacity. Your provider will discuss findings with you during a follow-up visit.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy