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Calcium Bilirubinate Crystals, Bile Fluid — Qualitative Microscopy

Published: August 9, 2026Updated: August 16, 2026

A microscopic examination of bile fluid that checks for calcium bilirubinate crystals, which may be associated with pigment-type gallstones.

Quick summary

["This test detects calcium bilirubinate crystals in bile, a finding that can occur with pigment stones or impaired bile flow."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
34471-3
Short code
Ca Bilirub Cry Bifl Ql Micro
Synonyms
Ca Bilirub Cry Bifl Ql Micro
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
microscopy-light
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This is a qualitative microscopy test of bile fluid. It checks whether calcium bilirubinate crystals, a type of pigment found in bile, are present.

Why might I need this test?

The test may be used when evaluating suspected gallbladder or bile duct disease, particularly when pigment-type gallstones, bile stasis, or obstruction is being considered. It is generally interpreted together with symptoms, imaging, and other laboratory findings.

How is the test performed?

A clinician or healthcare professional collects a small amount of bile fluid. A laboratory technician examines the specimen under a microscope and reports whether calcium bilirubinate crystals are detected.

How do I prepare for the test?

There is no universal preparation for a bile-fluid microscopy test. Follow the instructions provided by the team collecting the sample, especially if the specimen will be obtained during an endoscopic procedure.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Calcium Bilirubinate Crystals, Bile Fluid

Frequently asked questions

What does a positive result mean?
A positive result means calcium bilirubinate crystals were seen in the bile fluid. This can occur with pigment stones or impaired bile drainage but must be interpreted with the rest of the medical evaluation.
Does this test diagnose gallstones?
No. The finding may support a suspicion of pigment-type gallstones, but imaging and clinical information are usually needed to establish a diagnosis.
How is the bile-fluid sample obtained?
Bile may be collected from a drainage tube or aspirated during a procedure such as ERCP. The exact collection method depends on the clinical situation and is arranged by the healthcare team.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy