Skip to content

General Health Test Overview: Purpose, Preparation, Procedure, and How to Understand Your Medical Test Results

Published: July 31, 2026Updated: August 16, 2026

A test that measures the amount of calcium in your urine collected over a 12-hour period.

Quick summary

{"This test measures how much calcium your kidneys excrete in 12 hours","It helps diagnose conditions like kidney stones and hypercalciuria","Abnormal results may indicate kidney problems, bone disease, or parathyroid disorders","You will need to collect all urine for 12 hours in a special container","Certain medications and diet can affect your results"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
51610-4
Short code
CAU12
Synonyms
Calcium 12h Ur-sRate
Category
electrolyte
Sample
urine
Container
Sterile plastic urine container (24-hour or timed collection container)
Units
not specified
Ref low
1.25
Ref high
3.75
Critical range
6 not specified – 14 not specified
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Spectrophotometry (Arsenazo III method) or Atomic Absorption Spectroscopy (AAS)
CPT
82340
SNOMED
271565004
Specimen volume
Aliquot of 10-20 mL from complete 12-hour collection
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
24–72 hours
Order
routine

What is this medical test, and what specific health information can it provide about your body, your symptoms, or your ongoing treatment plan?

The 12-hour urine calcium test measures the amount of calcium eliminated from your body through urine over a 12-hour period. Calcium is an important mineral that helps build strong bones and teeth, supports muscle function, and helps nerves send signals. Your body normally balances calcium levels through absorption in the intestines, storage in bones, and excretion by the kidneys.

Why might your healthcare provider order this test, and how can the results help guide diagnosis, monitor a known condition, or assess whether treatment is working?

Your doctor may order this test if you have symptoms or risk factors for calcium-related conditions. It is commonly used to evaluate kidney stone formation risk, diagnose hypercalciuria (excessive calcium in urine), monitor parathyroid gland function, assess bone diseases, or track the effectiveness of certain treatments like diuretics or calcium supplements.

How should you prepare for this test, including whether you need to fast, adjust medicines, avoid activity, bring previous test results, or follow any special safety instructions?

Before the test, inform your doctor about all medications you are taking, as some drugs can affect calcium levels. You may be asked to avoid certain foods or supplements for 24 hours before collection. Your doctor may also provide specific dietary instructions. It is important to stay well-hydrated during the collection period unless otherwise instructed.

How is the test performed, what will happen step by step during the appointment, and what temporary sensations, risks, or aftereffects should you expect before or afterward?

You will receive a special container from the laboratory. On the morning of collection, urinate and discard that first sample. Note the exact time. For the next 12 hours, collect ALL urine in the container. At the end of 12 hours, urinate one final time and add that to the container. Keep the container refrigerated or in a cool place as instructed. Return the container to the lab as soon as possible after collection is complete.

Understanding your results means reviewing what the test found, comparing the findings with expected values or normal ranges, and discussing any follow-up tests, treatment options, or next steps with your healthcare provider.

Normal calcium excretion varies by laboratory, but typically ranges from 2.5 to 7.5 mmol per 24 hours. Your doctor will interpret your results based on your individual health situation. Low urine calcium may indicate vitamin D deficiency, hypoparathyroidism, or kidney problems. High urine calcium may suggest hypercalciuria, hyperparathyroidism, bone disease, or increased risk of kidney stones.

Reference range

1.25-3.75 mmol/12 hnot specified

Specimen: urine

Complete 12-hour timed urine collection required; empty bladder at start time and collect all urine thereafter; refrigerate during collection; no fasting needed but avoid calcium supplements if directed by physician

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

The 12-hour urine calcium test is clinically significant for diagnosing and managing several conditions. Elevated urine calcium (hypercalciuria) is a major risk factor for calcium kidney stones, affecting approximately 10% of the population. The test helps identify individuals at risk for stone formation so preventive measures can be taken. It also aids in distinguishing between different causes of abnormal calcium metabolism, including primary hyperparathyroidism, certain bone disorders, and renal tubular defects.

This test is also valuable for monitoring patients on certain medications that affect calcium excretion, such as thiazide diuretics (which decrease calcium excretion) or loop diuretics (which increase it). In patients with osteoporosis, the test can help assess calcium balance and guide treatment decisions. Additionally, it may be used to evaluate response to dietary modifications or medication therapy aimed at reducing stone recurrence.

Frequently asked questions

Does this test require fasting?
Fasting is not typically required for the urine collection itself, but you may be asked to follow specific dietary instructions for 24 hours before starting collection. Follow your doctor's orders regarding food and drink restrictions.
Can I collect urine at home?
Yes, urine collection is done at home using a special container provided by the laboratory. You will need to keep the container refrigerated or in a cool place during the 12-hour collection period and return it to the lab promptly after completion.
What happens if I miss some urine during the collection?
It is crucial to collect ALL urine during the 12-hour period for accurate results. If you miss a sample, note the time and try to collect the next one. Contact your healthcare provider or laboratory for guidance if you accidentally miss part of the collection.
Are there any risks with this test?
There are no significant risks from the test itself. It is a simple urine collection procedure. However, be sure to wash your hands thoroughly after handling the collection container and avoid touching the inside of the container to prevent contamination.
How long does it take to get results?
Results are typically available within 1-3 days after the sample reaches the laboratory. Your doctor will contact you with the results or discuss them at your follow-up appointment.
Should I stop taking my medications before the test?
Do not stop taking any prescribed medications unless your doctor specifically instructs you to do so. Certain medications can affect test results, and your doctor needs to know what you are taking to interpret the results correctly. Always consult your healthcare provider before making any changes to your medication regimen.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy