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Overview of This Medical Test: What It Is, Why It May Be Needed, How It Is Performed, and How You Should Prepare Before Your Appointment

Published: July 31, 2026Updated: August 16, 2026

A test that measures the amount of calcium in your urine collected over 18 hours to evaluate calcium metabolism and kidney function.

Quick summary

{"This test measures calcium excretion in urine collected over 18 hours","It helps diagnose conditions like kidney stones and parathyroid disorders","You will need to collect all urine during the 18-hour period","Certain medications and diet can affect test results","Results are compared with blood calcium levels for complete assessment"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59000-0
Short code
CAU18
Synonyms
Calcium 18h Ur-mRate
Category
electrolyte
Sample
urine
Container
24-hour urine collection container (plastic, graduated)
Units
not specified
Ref low
75
Ref high
225
Critical range
6 not specified – 14 not specified
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
Atomic Absorption Spectrophotometry (AAS) or Spectrophotometry using Arsenazo III dye
CPT
82340
SNOMED
271568004
Specimen volume
Entire 18-hour urine collection (typical volume 800-2000 mL)
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
24–72 hours
Order
routine

What is this test? This medical test is a clinical evaluation used to help your provider learn more about your symptoms, monitor your health, or guide decisions about treatment and follow-up care.

The 18-hour urine calcium test measures the amount of calcium your kidneys excrete in urine over an 18-hour period. Calcium is an essential mineral important for bone health, nerve function, and muscle contraction. This test helps doctors understand how your body processes calcium and how well your kidneys are working.

How is the test performed? A trained staff member may take a blood sample, collect urine or saliva, swab a body area, or use imaging or physical exam steps. The exact process depends on the test your provider ordered and where you go for care.

You will receive a special container for urine collection. Typically, you start collecting urine in the morning after the first morning void and continue collecting for the next 18 hours, including the first morning void on the second day. It is important to collect all urine during this period for accurate results. The collected urine is then sent to a laboratory for analysis.

How should you prepare for the test? Follow the instructions given to you, such as fasting for a set number of hours, drinking water, avoiding exercise, or discussing current medicines. Ask your provider or lab staff before changing or stopping any prescribed medicine.

Your doctor may ask you to maintain your normal diet for a few days before the test. Continue taking your regular medications unless instructed otherwise. Some medications can affect calcium levels, so inform your doctor about all medications and supplements you are taking. Drink adequate fluids during the collection period unless otherwise advised.

Reference range

75-225 mg/18 hnot specified

Specimen: urine

Complete timed 18-hour urine collection required (discard first void); no fasting typically needed.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

The 18-hour urine calcium test is primarily used to diagnose and monitor hypercalciuria (excessive calcium in urine), which is a major risk factor for kidney stones. It also helps evaluate parathyroid function, bone diseases, and certain kidney disorders. High urine calcium levels may indicate hyperparathyroidism, vitamin D excess, or certain cancers. Low urine calcium levels may be seen in hypoparathyroidism, vitamin D deficiency, or chronic kidney disease.

Frequently asked questions

Why do I need to collect urine for 18 hours?
A single urine sample may not accurately reflect your calcium excretion because calcium levels vary throughout the day. An 18-hour collection provides a more reliable average measurement.
What happens if I miss collecting some urine during the 18 hours?
It is important to collect all urine during the collection period. If you miss a collection, inform your healthcare provider. They may ask you to restart the collection or adjust the interpretation of results.
Are there any risks associated with this test?
There are no significant risks from the test itself. The main challenge is ensuring accurate urine collection. If you have a catheter or difficulty with collection, discuss alternatives with your doctor.
How long does it take to get results?
Results are typically available within a few days after the sample reaches the laboratory. Your healthcare provider will discuss the results with you and explain what they mean for your health.
Do I need to fast before this test?
Fasting is usually not required for a urine calcium test. However, follow any specific instructions given by your healthcare provider regarding diet or medications before the test.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy