Canary Yellow Serum Protein Electrophoresis (Bromocresol Green Method)
This test measures the distribution of different protein fractions in your blood serum using a dye called bromocresol green. It helps identify patterns of protein separation that may indicate conditions affecting protein synthesis, breakdown, or transport.
["This test helps doctors assess protein balance in your blood, often used to check for liver disease, kidney problems, or other metabolic disorders."]
Key facts at a glance
- LOINC
- 31280-1
- Short code
- CANSP
- Synonyms
- Canary Serum Prot Ab Ql
- Category
- chemistry
- Sample
- serum
- Container
- Serum separator tube (SST) or red-top tube with clot activator
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- Enzyme-linked immunosorbent assay (ELISA) or Immunofluorescence assay (IFA)
- CPT
- 86003
- SNOMED
- 39513008
- Specimen volume
- 1-2 mL of serum
- Stability
- Room temperature: 8 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C or below): 30 days
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 48–96 hours
- Order
- routine
What This Test Measures
The bromocresol green dye binds to proteins in your serum, causing them to separate into distinct bands during electrophoresis. Each band represents a group of proteins with similar molecular weights. The dye’s color change helps visualize these fractions, including albumin, globulins, and other protein components.
Clinical Uses
This test is primarily used to diagnose or monitor conditions like chronic liver disease, nephrotic syndrome, multiple myeloma, or other disorders that alter protein production or distribution in the blood.
How the Test Works
A small blood sample is drawn and mixed with bromocresol green. The sample is then placed in an electric field, causing proteins to migrate based on their size and charge. The resulting pattern of bands is analyzed to determine protein concentration and distribution.
Preparation for the Test
No special preparation is needed, but avoid heavy meals or excessive alcohol before the test. Fasted or non-fasted states may not significantly affect this test, but hydration is important.
Reference range
Negative (no detectable antibodies to canary serum proteins); Positive results indicate sensitization or exposureN/A
No fasting required. Patients should inform the laboratory of any current antihistamine or corticosteroid use, as these may affect IgE antibody levels. Avoid recent allergen exposure if clinically feasible prior to testing.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Canary Yellow Serum Protein Electrophoresis
Frequently asked questions
Why might my doctor order this test?
What does a changed result mean?
How often should I get this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy