Prostate-Specific Antigen (PSA) with Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Quantification
This test measures the level of prostate-specific antigen (PSA), a protein produced by both healthy and cancerous prostate cells. It helps assess prostate health and detect potential prostate issues.
["PSA levels can guide discussions about prostate health, including screening or monitoring for prostate cancer."]
Key facts at a glance
- LOINC
- 19187-4
- Short code
- CA2729PF
- Synonyms
- Cancer Ag27-29 Plr-aCnc
- Category
- chemistry
- Sample
- fluid
- Container
- Sterile, leak-proof, screw-cap collection container or red-top vacuum tube; avoid additives when possible. Heparinized tubes may be used if small volume is collected.
- Units
- N/A
- Ref low
- 27
- Ref high
- 29
- Critical range
- 0.5 N/A – —
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 18 years
- Method
- Immunoassay (Chemiluminescent or Radioimmunoassay)
- CPT
- 86316
- SNOMED
- 395124004
- Specimen volume
- 2-5 mL of pleural fluid
- Stability
- Refrigerate at 2-8°C; analyze within 24 hours of collection. For prolonged storage, freeze at -20°C or lower.
- Hemolysis max
- 300
- TAT
- 24–72 hours
- Order
- routine
Your doctor may order this test to help diagnose a medical condition, monitor an ongoing health problem, or check how well a treatment is working. Understanding why the test has been recommended can help you feel more prepared and confident as you take part in your own care.
Doctors may order this test to monitor prostate health, especially if you have symptoms like urinary issues, pain in the pelvic area, or a family history of prostate cancer. It can also be used during routine check-ups or after a prostate biopsy.
This test works by analyzing a sample in a laboratory, where trained professionals measure specific substances or markers in your body. The results give your healthcare team important information about your health that cannot be seen during a routine physical examination alone.
This test uses a highly sensitive method called liquid chromatography-tandem mass spectrometry (LC-MS/MS) to precisely measure PSA levels in your blood. Unlike traditional PSA tests, this method provides more accurate and detailed results.
A sample will be collected either through a small blood draw in your arm or from another type of specimen, depending on what your doctor has ordered. A trained technician will gather the sample quickly and safely, and it will then be sent to the laboratory for careful analysis.
A blood sample is drawn from a vein, usually in your arm. The blood is then sent to a lab for testing. This process is quick and typically done in a doctor’s office or clinic.
Before your test, your healthcare provider will explain the procedure and tell you whether you need to fast, avoid certain medications, or follow other special instructions. Be sure to mention all medicines, vitamins, and supplements you take so your results are as accurate as possible.
No special preparation is usually needed, but you may be asked to avoid ejaculation for 48 hours before the test if it’s part of a screening or monitoring plan. Always follow your healthcare provider’s instructions.
Reference range
Cancer Ag 27-29 is typically not detectable in normal pleural fluid. Reference ranges vary by assay manufacturer; consult laboratory-specific reference values. Generally, pleural fluid levels are interpreted alongside serum levels and clinical context.N/A
No special patient preparation required prior to pleural fluid collection (thoracentesis).
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
PSA LC-MS/MS
Frequently asked questions
PSA levels can vary based on factors like age, prostate size, and overall health. While elevated PSA can signal prostate cancer or other conditions (like benign prostatic hyperplasia), it’s not definitive on its own. Results are often interpreted alongside clinical findings and patient history.
What should I do if I still have questions about my test or my results?
Can PSA levels be affected by other factors besides prostate cancer? Yes, conditions like prostate inflammation, infections, or benign growths can also influence PSA levels. Your doctor will consider these factors when interpreting your results.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy