Multigene Cancer Analysis Using Plasma Cell-Free DNA (cfDNA)
This test analyzes fragments of DNA found naturally in your blood plasma to check for genetic changes associated with certain cancers. It helps identify mutations linked to cancer risk or progression, often used in precision oncology.
["This test may help guide treatment decisions by detecting cancer-related genetic mutations in your blood."]
Key facts at a glance
- LOINC
- 100029-8
- Short code
- CFDNA PANEL
- Synonyms
- Cancer multigene analysis Plas.cfDNA
- Category
- chemistry
- Sample
- plasma
- Container
- Streck Cell-Free DNA BCT (or equivalent cfDNA-preserving blood collection tube) — 10 mL tubes, typically 2 tubes collected (minimum 1 tube required). EDTA plasma tubes are not preferred but may be acceptable with rapid processing.
- Units
- N/A
- Ref low
- 136
- Ref high
- 145
- Critical range
- 120 N/A – 160 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–120 years
- Method
- molgen
- CPT
- 81445, 81455, 81462, 81463, 81464 (may also include 81235 for EGFR, 81210 for BRAF, 81275 for KRAS depending on panel composition and individual gene billing)
- SNOMED
- 426396006
- Specimen volume
- 8-10 mL of whole blood collected into cfDNA-stabilizing tubes (minimum 2 tubes; approximately 20 mL total recommended for adequate cfDNA yield)
- Stability
- Whole blood in cfDNA preservation tubes: stable at room temperature (15-25°C) for up to 7 days per manufacturer (Streck BCT); do not refrigerate or freeze whole blood. Plasma separated from cells should be stored at 2-8°C if processed within 24 hours, or frozen at -80°C for longer storage (up to 30 days) prior to cfDNA extraction.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 120–336 hours
- Order
- routine
What Is Being Tested?
The test looks for specific genetic mutations (called *mutations*) in tiny pieces of DNA (called *cell-free DNA*, or cfDNA) floating in your blood plasma. These mutations are often linked to cancer development or progression. The test checks for changes in multiple genes known to be involved in cancer, such as those involved in DNA repair, cell growth, or signaling pathways.
Why Might This Test Be Ordered?
Your healthcare provider may recommend this test if you have a known cancer history, are at high risk for certain cancers, or are being evaluated for treatment options. It can also be used in monitoring cancer after treatment to check for recurrence.
How Is the Sample Collected?
A blood sample is drawn from a vein in your arm. The plasma portion of your blood is separated and analyzed for small DNA fragments. This process is non-invasive and typically done in a lab setting.
What Are the Possible Results?
Results may indicate the presence of specific genetic mutations associated with cancer. If mutations are found, they can help determine the type of cancer, guide treatment choices, or assess how well your cancer is responding to treatment. Results may also be negative if no cancer-related mutations are detected in the tested genes.
Reference range
Adult: 136-145 mmol/LN/A
Fasting is not required. Patients should be informed that this test analyzes tumor-derived DNA circulating in the bloodstream (liquid biopsy) and is not a substitute for tissue biopsy in all clinical contexts. Recent surgery, blood transfusion, or hematopoietic stem cell transplantation within the prior 2-4 weeks may yield false-positive or false-negative results due to donor-derived cfDNA.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test provides critical information for personalized cancer care. By identifying specific genetic mutations, doctors can tailor treatments to target the cancer’s underlying biology, potentially improving outcomes. It may also help monitor cancer progression or response to therapy by detecting changes in the genetic material over time.
Short name
Plasma cfDNA Cancer Panel
Frequently asked questions
How long will it take to get my results?
What if my test results are positive for cancer-related mutations?
No, this test focuses on specific genes associated with certain cancers. It may not detect all types of cancer, especially those without the same genetic mutations being tested. Your provider will clarify which cancers this test is designed to identify.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy