Borrelia burgdorferi IgG Antibody Titration by Immunofluorescence
This test measures the level of antibodies to *Borrelia burgdorferi*, the bacterium responsible for Lyme disease, using a fluorescent antibody technique. It helps determine if you’ve been exposed to the infection and whether treatment may be needed.
["This test helps diagnose Lyme disease by checking for past or current exposure to the Lyme bacteria."]
Key facts at a glance
- LOINC
- 38389-3
- Short code
- COXPH2
- Synonyms
- C burnet Ph2 Ab Titr Ser
- Category
- chemistry
- Sample
- serum
- Container
- Serum separator tube (SST) or red-top tube
- Units
- N/A
- Ref low
- 0
- Ref high
- 1
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- Immunofluorescence Assay (IFA)
- CPT
- 86638
- SNOMED
- 115329005
- Specimen volume
- 1-2 mL serum
- Stability
- Stable for 7 days at 2-8°C; for extended storage, freeze at -20°C or below
- Hemolysis max
- 100
- Lipemia max
- 100
- Icterus max
- 50
- TAT
- 48–96 hours
- Order
- routine
Why This Test Matters
Lyme disease is spread through the bite of infected ticks. If you’ve had symptoms like fever, rash, or joint pain—especially after being outdoors in grassy or wooded areas—this test can help confirm whether you’ve been exposed to *Borrelia burgdorferi*. Early detection is key to managing symptoms and preventing long-term complications.
How the Test Works
Your blood sample is tested using immunofluorescence, where antibodies specific to *Borrelia burgdorferi* are detected and measured. The result is expressed as a titer (a numerical value indicating antibody concentration), which helps doctors assess exposure level.
What to Expect Before the Test
No special preparation is needed. Bring any relevant medical history or symptoms to your doctor’s visit. If you’re taking antibiotics, let your provider know—it may affect results.
What the Results Mean
A positive result means you likely had exposure to *Borrelia burgdorferi* at some point. A negative result suggests no detectable antibodies, but it doesn’t rule out past exposure. Follow-up testing or clinical evaluation may be needed if symptoms persist.
Reference range
Negative: <1:16; Equivocal: 1:16; Positive: ≥1:32. Phase 2 antibodies indicate exposure or early/convalescent infection with Coxiella burnetii (Q fever).N/A
No special patient preparation required. Fasting is not necessary. Acute and convalescent serum samples are recommended for accurate diagnosis.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Borrelia IgG Antibody Titer
Frequently asked questions
If you’ve had symptoms like a bullseye rash (erythema migrans), fatigue, or neurological issues after a tick bite, this test helps doctors diagnose Lyme disease. It’s also used to monitor treatment progress or rule out other infections.
How long will I get my results?
Can I get this test at home?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy