Skip to content

Expression and Functional Analysis of Prolymphocytic Leukemia Cells (FC)

Published: August 9, 2026Updated: August 16, 2026

This test evaluates how well your blood cells involved in prolymphocytic leukemia function and express certain markers, which may help guide treatment decisions.

Quick summary

["Assists doctors in understanding leukemia cell behavior and treatment response."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
98239-7
Short code
CD154FC
Synonyms
CD154 Expr+Func Pnl T cells FC
Category
chemistry
Sample
serum
Container
Sodium heparin (green-top) or ACD (acid-citrate-dextrose) anticoagulated whole blood tube. EDTA tubes are acceptable for immunophenotyping but not preferred for functional assays. Sterile collection required to maintain cell viability.
Units
N/A
Ref low
0
Ref high
2
Fasting
false
Population
adult
Sex
all
Age range
0–99 years
Method
flow-cytometry
CPT
88184, 88185 (flow cytometry panels); 86359 (T cell functional assay); 88187-88189 (interpretation as applicable)
SNOMED
428481001, 313275009
Specimen volume
3-5 mL whole blood for adults; 1-3 mL for pediatric patients. Minimum 1 mL required. Bone marrow samples may require different volume specifications.
Stability
Specimen should be processed within 24-48 hours of collection when stored at room temperature (18-25°C). Do not refrigerate or freeze whole blood. For functional assays, process within 24 hours for optimal results. Activated samples may be fixed and stored for delayed analysis.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
24–72 hours
Order
routine

What This Test Measures

This test assesses the expression of specific proteins and functional activity in prolymphocytic leukemia cells. It may include measurements of cell viability, proliferation rates, and marker presence (e.g., CD10, CD25) to detect abnormalities.

Why It’s Ordered

Your healthcare provider may order this test if you have symptoms of leukemia (e.g., fatigue, weight loss) or if you’re undergoing treatment to monitor effectiveness or detect resistance.

Sample Collection and Preparation

A blood sample is taken from a vein, typically in a clinic or lab. The cells are processed to isolate prolymphocytic leukemia cells for analysis, often using specialized techniques like flow cytometry or functional assays.

What to Expect Before the Test

No special preparation is usually needed, but inform your provider about medications (e.g., blood thinners) or recent illnesses that might affect results.

Reference range

CD154 expression on resting T cells: <2% of CD4+ T cells. After activation (PMA/ionomycin): ≥60% of CD4+ T cells express CD154. Functional response is reported as percentage of CD154+ CD4+ T cells and median fluorescence intensity (MFI). Reference ranges established by each laboratory; consult institutional reference values.N/A

Specimen: serum

No fasting required. Patients should be well-hydrated. Avoid recent immunosuppressive therapy when possible, as it may affect T cell function results. Inform the laboratory of any current medications including corticosteroids, calcineurin inhibitors, biologics, or chemotherapy agents that may impact immune cell function.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Prolymphocytic Leukemia Cell Function Test

Frequently asked questions

How long does it take to get results?
Results are typically available within 1–2 weeks, depending on lab processing and specialized testing requirements.
What does an abnormal result mean?
An abnormal result may indicate persistent or worsening leukemia activity, resistance to treatment, or specific genetic traits that could influence future care. Always discuss findings with your doctor.
Can lifestyle changes affect this test?
Generally, no lifestyle changes are expected to impact this test, but avoid strenuous activity or medications that may alter blood cell function 24 hours before testing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy