CD19+CD10+ B Cells in Whole Blood
This test identifies and counts B cells in a blood sample that carry both the CD19 and CD10 surface markers, which helps characterize certain immune and blood conditions.
["CD19+CD10+ B cells are a normally small B-cell subset; measuring them helps doctors evaluate the immune system and investigate some blood cancers."]
Key facts at a glance
- LOINC
- 60434-8
- Short code
- CD19CD103
- Synonyms
- CD19+CD103+ Cells # Bld
- Category
- chemistry
- Sample
- whole-blood
- Container
- Lavender-top (EDTA) anticoagulant tube is preferred for flow cytometry immunophenotyping. ACD (yellow-top) or sodium heparin (green-top) tubes may also be acceptable depending on laboratory protocol.
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- Flow cytometry using fluorescein (FITC) and phycoerythrin (PE) or other fluorochrome-conjugated monoclonal antibodies against CD19 and CD103 surface antigens, followed by acquisition on a clinical flow cytometer and absolute count calculation using counting beads or dual-platform methodology with concurrent complete blood count (CBC) with differential.
- CPT
- 86356 (Monoclonal antibody, flow cytometry, each additional marker); may be billed in conjunction with 86355 for the primary marker or as part of 86360 (absolute CD4/CD8 count with ratio) when applicable
- SNOMED
- 399142005 (Measurement of lymphocyte subset); may vary by performing laboratory
- Specimen volume
- Minimum 3 mL of whole blood for adults; minimum 1 mL for pediatric patients. Adequate sample is required to obtain sufficient events for accurate rare-event analysis.
- Stability
- Specimen should be processed within 24 to 48 hours of collection when stored at room temperature (18–25 °C). Prolonged storage beyond 48 hours may result in loss of cell viability and altered surface antigen expression. Do not refrigerate or freeze whole blood specimens intended for flow cytometry.
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 24–72 hours
- Order
- routine
What This Test Measures
B cells are a type of white blood cell that make antibodies and help fight infection. Each B cell has surface proteins called markers that let laboratory instruments identify it. CD19 is a marker found on most B cells, and CD10 is a marker seen on immature B cells. This test uses flow cytometry, a method that labels cells with fluorescent tags and counts them one by one, to identify B cells that carry both markers and report how many are present.
Why Your Doctor Ordered It
Your doctor may order this test to evaluate the immune system or to investigate an abnormal complete blood count. It is often part of a panel that describes the different B-cell populations in your blood. The CD19+CD10+ pattern can be useful when certain lymphomas or other blood disorders are suspected, and it may also be checked during monitoring of a known condition.
How the Sample Is Collected
A small amount of whole blood is drawn from a vein, usually in your arm, and placed in a tube suitable for flow cytometry. The sample is sent to a specialized laboratory where the cells are stained with antibodies that recognize CD19 and CD10 and then run through the analyzer.
What the Results Mean
The result reports the percentage or number of B cells that carry both markers. Small amounts of CD19+CD10+ B cells can be normal. Increased or unusual patterns must be interpreted together with your symptoms, blood counts, and other tests. Your care team will explain what your specific result means, because a single number does not make a diagnosis on its own.
Reference range
Reference ranges for CD19+CD103+ co-expressing B lymphocytes in peripheral blood are not well-established in standard clinical literature. CD19+CD103+ cells represent a rare B-cell subset, typically comprising a very small fraction of circulating B lymphocytes. Values are laboratory-specific; results should be interpreted using the performing laboratory's established reference interval. Studies in healthy adults generally report very low or undetectable levels of CD19+CD103+ cells in peripheral blood.N/A
No special patient preparation is required. Fasting is not necessary. Patients should be well-hydrated. Recent corticosteroid therapy, chemotherapy, immunotherapy, or immunosuppressive medications may affect lymphocyte subset distribution and should be noted on the requisition.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
CD19+CD10+ B cells are an immunophenotypic subset assessed by flow cytometry. Their measurement supports evaluation of normal and abnormal B-cell populations, including conditions such as some B-cell lymphomas and other lymphoproliferative or immune disorders. Results are interpreted in the context of the complete blood count, blood smear, clinical history, and sometimes additional markers, so this test is one piece of a larger evaluation rather than a stand-alone diagnosis.
Short name
CD19/CD10 B-Cell Subset
Frequently asked questions
Does an abnormal result mean I have cancer?
Is this test the same as a regular B-cell count?
Do I need special preparation before the blood draw?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
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Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy