: CD19 and CD58 Cell Surface Marker Evaluation, Body Fluid
: This test uses flow cytometry to identify and count cells in a body fluid sample that carry the CD19 and CD58 surface markers. It is used to help characterize certain immune and blood cell populations.
[": It measures CD19- and CD58-positive cells in a body fluid to support evaluation of immune or blood cell disorders."]
Key facts at a glance
- LOINC
- 51355-6
- Short code
- CD19CD58BF
- Synonyms
- CD19+CD58+ Cells NFr Fld
- Category
- chemistry
- Sample
- serum
- Container
- Sterile, anticoagulated collection tube (e.g., sodium heparin or EDTA tube) appropriate for flow cytometry. For cerebrospinal fluid, a sterile polypropylene tube is preferred. Containers should be labeled with patient identifiers, specimen source, and collection time.
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- Flow cytometry using fluorescently labeled monoclonal antibodies against CD19 and CD58 surface markers, with subsequent enumeration of positive cells as a percentage of total lymphocytes or absolute count per unit volume.
- CPT
- 88184
- SNOMED
- 395124004
- Specimen volume
- Minimum 1 mL of body fluid is generally required, though larger volumes (e.g., 3-5 mL of CSF) improve cell yield and detection sensitivity for rare populations. Volume requirements may vary based on cell concentration and clinical priority.
- Stability
- Specimens should be processed as soon as possible, ideally within 4 hours of collection when maintained at room temperature (18-25°C). Refrigeration (2-8°C) may be acceptable for up to 24 hours for some fluid types, but viability and antigen expression may decline with prolonged storage.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–48 hours
- Order
- routine
: What This Test Measures
: The test detects cells expressing CD19, a marker found mainly on B lymphocytes, and CD58, a surface protein present on many immune and other cells. Flow cytometry is used to sort and count cells carrying these markers in the fluid sample provided.
: Why It May Be Ordered
: Your clinician may order this test to investigate an abnormal cell population, evaluate a suspected lymphoma or other blood/immune condition, or monitor a known disorder involving B cells or other CD58-expressing cells. It is often part of a broader panel of marker studies.
: How the Sample Is Collected
: A sample of body fluid (such as pleural, peritoneal, or cerebrospinal fluid) is collected by your healthcare provider using standard sterile technique. The sample is then sent to the laboratory for flow cytometric analysis.
: What the Results Mean
: Results describe the presence and proportion of CD19- and CD58-positive cells. Your provider interprets these findings together with your symptoms, other lab tests, and imaging. Atypical patterns may prompt additional testing; normal patterns help rule out certain conditions.
Reference range
Reference ranges are laboratory-specific and should be interpreted in conjunction with clinical findings. In healthy individuals, CD19+CD58+ cells are typically present in low proportions within peripheral blood and body fluids. Results should be compared against validated laboratory reference intervals or published literature for the specific body fluid analyzed.N/A
No special patient preparation is required. However, relevant clinical history, including suspected diagnosis, prior treatments (such as chemotherapy or immunotherapy), and information about specimen source, should be provided to the laboratory to ensure appropriate interpretation and gating strategies during flow cytometric analysis.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
: CD19 and CD58 marker analysis can help distinguish between reactive and neoplastic cell populations and support diagnosis or monitoring of B-cell and other immune-related disorders. Results should always be interpreted in the context of the full clinical picture and other laboratory findings.
Short name
: CD19/CD58 Flow Cytometry
Frequently asked questions
: Does this test diagnose cancer on its own?
: Is the procedure painful?
: Do I need to fast before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy