CD27 and CD19 Immunophenotyping of Immature B-Cell Populations in Blood
This test measures specific immune cell markers (CD27 and CD19) in your blood to help evaluate the presence of immature B-cells, which may be relevant in conditions affecting immune development or disorders like leukemia.
["Assists in diagnosing or monitoring blood disorders involving immature B-cells."]
Key facts at a glance
- LOINC
- 80706-5
- Short code
- CD27IGD
- Synonyms
- CD27+IgD- cells/Cells.CD19+CD20+ NFr Bld
- Category
- chemistry
- Sample
- whole-blood
- Container
- EDTA anticoagulant tube (lavender/purple-top) for whole blood flow cytometry; sodium heparin tube (green-top) acceptable alternative
- Units
- N/A
- Ref low
- 6
- Ref high
- 29
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–99 years
- Method
- Flow cytometry using fluorescently-labeled monoclonal antibodies against CD27, IgD, CD19, and CD20 surface markers; results expressed as percentage of CD19+CD20+ B cells expressing CD27 but lacking surface IgD (class-switched memory B cells)
- CPT
- 86356 (single antigen); 86355 (comprehensive flow cytometry panel)
- SNOMED
- 122555000
- Specimen volume
- Minimum 1-3 mL whole blood for adult tube; pediatric microtainer minimum 0.5 mL
- Stability
- Specimen should be processed within 24-48 hours of collection when stored at room temperature (18-25°C). Do not refrigerate whole blood as this may affect cell viability and surface marker expression. If processing is delayed, use validated stabilization protocols per laboratory SOP.
- Hemolysis max
- 100
- Lipemia max
- 300
- Icterus max
- 20
- TAT
- 24–72 hours
- Order
- routine
What This Test Measures
CD27 and CD19 are proteins found on the surface of certain immune cells. CD19 is a marker present on all mature B-cells and some immature precursors, while CD27 is typically found on more mature B-cells. This test detects the presence and proportion of cells that express these markers in a sample of your blood, specifically focusing on immature B-cell populations.
Why It Might Be Ordered
Your healthcare provider may order this test if you have symptoms or a history suggestive of conditions like B-cell leukemia, lymphoma, or congenital immune deficiencies. It can also be used to monitor treatment response in patients receiving therapies that target immature blood cells.
Sample Collection and Preparation
A small amount of your blood will be drawn into a special tube. The blood sample is then processed to separate and analyze specific cell populations using advanced laboratory techniques, such as flow cytometry, to identify cells expressing CD27 and CD19.
What to Expect Before the Test
No special preparation is typically required. You may have some mild discomfort from the needle, but it usually goes away quickly. If you are on blood-thinning medication, inform your provider before the test.
Reference range
Typically reported as percentage of class-switched memory B cells (CD27+IgD-) within the total CD19+CD20+ B cell population. Reference intervals vary by laboratory; commonly reported adult ranges are approximately 6-29% of total B cells. Pediatric reference values are age-dependent and generally higher in young children, declining to adult levels by adolescence.N/A
No special fasting or dietary restrictions required. Patients should be well-hydrated. Inform the laboratory of any recent immunosuppressive therapy, rituximab or other anti-CD20 treatment, chemotherapy, or corticosteroid use, as these can transiently alter B cell subsets.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal levels of immature B-cells detected by this test can indicate underlying hematologic disorders, such as acute lymphoblastic leukemia (ALL) or other congenital or acquired immune system abnormalities. It helps guide treatment decisions and assesses disease progression or response to therapy by providing insight into the presence and behavior of these cells in your bloodstream.
Short name
B-Cell Immature Subset Analysis
Frequently asked questions
Will this test show if I have a blood cancer?
How long will it take to get my results?
Can I eat or drink before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy