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Absolute Count of CD3+ Cells in Lymph Nodes (Biopsy)

Published: August 9, 2026Updated: August 16, 2026

This test measures the number of CD3+ T cells in a sample of lymph node tissue, which helps assess immune cell activity in the affected area.

Quick summary

["Understanding your T-cell count in lymph nodes can help diagnose or monitor conditions affecting your immune system."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
103682-1
Short code
CD3CD25BAL
Synonyms
CD3+CD25+ Cells/lymph NFr BAL
Category
chemistry
Sample
serum
Container
Sterile BAL collection container; transfer to tubes containing appropriate anticoagulant (heparin or EDTA) for flow cytometry. Maintain at room temperature.
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
Flow Cytometry (Immunophenotyping)
CPT
86359 (T cells, total count); 86360 (T cells, absolute CD4 and CD8 count, including ratio); additional flow cytometry codes may apply
SNOMED
122556008 |Bronchoalveolar lavage specimen|; 313276009 |Lymphocyte subset analysis|
Specimen volume
Minimum 5-10 mL of BAL fluid required for lymphocyte subset analysis; optimal volume 20-50 mL
Stability
Process within 2-4 hours of collection for optimal cell viability. If delay expected, refrigerate at 2-8°C; do not freeze. Samples should be analyzed within 24 hours of collection.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
24–72 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like persistent lymph node swelling, unexplained weight loss, or signs of an immune-related condition. It can also be part of a biopsy to evaluate suspected lymphomas, infections, or autoimmune diseases.

What to Expect Before the Test

No special preparation is typically needed beyond standard biopsy protocols. You may be asked to fast or avoid certain medications, but this depends on your specific procedure instructions.

What the Test Involves

A small sample of lymph node tissue is collected through a biopsy. The tissue is then processed in the lab to count CD3+ T cells, which are a type of white blood cell critical for immune function.

How Results Are Interpreted

Results are compared to expected ranges for healthy tissue, but exact values depend on the lab and clinical context. Abnormal counts may suggest conditions like lymphoma, chronic infections, or autoimmune disorders.

Reference range

Reference values for CD3+CD25+ lymphocytes in bronchoalveolar lavage (BAL) fluid are not well standardized. Typically expressed as a percentage of total lymphocytes or as absolute count per mL of BAL fluid. Consult laboratory-specific reference ranges.N/A

Specimen: serum

Patient should fast for at least 4-6 hours prior to bronchoscopy procedure. Inform physician of all current medications, especially immunosuppressants, corticosteroids, and anticoagulants. Conscious sedation may be administered; arrange for transportation home after procedure.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CD3+ Lymphocyte Count (Lymph Node)

Frequently asked questions

What does an abnormal CD3+ lymphocyte count in lymph nodes mean?
An elevated count might indicate chronic inflammation, infections, or certain lymphomas. A reduced count could suggest immune suppression, advanced disease, or other systemic conditions. Always discuss results with your doctor for personalized interpretation.
How often will I need this test?
Results may guide follow-up testing or treatment adjustments. Your provider will determine if repeat testing is needed based on your symptoms, response to treatment, or ongoing evaluation of your condition.
Can lifestyle changes affect this test result?
While diet, stress, or medications may influence immune cell activity, this specific test focuses on tissue samples. Your provider will consider all relevant factors when reviewing results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy