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Serum or plasma concentration of vitamin D (25-hydroxyvitamin D)

Published: August 9, 2026Updated: August 15, 2026

This test measures the amount of vitamin D in your blood, which helps your body absorb calcium and maintain bone health. Low levels may indicate deficiency, while high levels could signal overabsorption or other conditions.

Quick summary

["Vitamin D levels help assess bone health and overall nutrient status."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 15, 2026Editorial policy

Key facts at a glance

LOINC
96537-6
Short code
CEVIGM
Synonyms
CEV IgM Spec Ql IA
Category
chemistry
Sample
serum
Container
Serum separator tube (SST) or red-top tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
Enzyme Immunoassay (EIA)
CPT
86651
SNOMED
39529009
Specimen volume
1-2 mL serum
Stability
Serum stable for 7 days at 2-8°C; for longer storage, freeze at -20°C or below
Hemolysis max
100
Lipemia max
100
Icterus max
50
TAT
24–72 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to check if you have enough vitamin D, which is important for bone strength, immune function, and muscle health. It’s often done if you have symptoms like muscle weakness, frequent infections, or conditions like osteoporosis.

What to Expect Before the Test

No special preparation is needed unless instructed. You may fast before the test if directed, but always follow your provider’s advice. Bring any medications you’re taking, as some can affect vitamin D levels.

What Happens During the Test?

A small needle will draw blood from a vein in your arm. The process is quick and usually causes only mild discomfort. The blood sample is sent to a lab for analysis.

What Are the Possible Results?

Results are reported as a concentration of vitamin D in the blood, measured in nanograms per milliliter (ng/mL). Your provider will interpret the results in relation to your health and symptoms.

Reference range

Negative: No California encephalitis virus IgM antibodies detected. Positive: IgM antibodies to California encephalitis virus detected, suggesting recent or current infection.N/A

Specimen: serum

No fasting or special preparation required. Specimen should be collected during the acute phase of illness for optimal IgM antibody detection.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Vitamin D level

Frequently asked questions

How long does it take to get my results?
Results are typically available within 1 to 3 business days, depending on your lab’s processing time. Your healthcare provider will contact you once they are ready.
What does a low vitamin D level mean?
A low vitamin D level suggests you may not be getting enough from sunlight, diet, or supplements. This can affect bone health and may contribute to other health issues like fatigue or weakened immunity. Your provider may recommend increasing sun exposure, dietary changes, or supplements to raise your levels.
Can vitamin D levels change over time?
Yes, vitamin D levels can fluctuate based on factors like sun exposure, diet, age, and overall health. Seasonal changes, medications, and chronic conditions can also influence them. Regular monitoring may be needed if you have ongoing health concerns or take supplements.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy