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Cardiolipin Antibody Total (IgG) – Donor-Specific

Published: August 9, 2026Updated: August 15, 2026

This test measures total IgG antibodies against cardiolipin, a phospholipid found in cell membranes, often used to assess for lupus anticoagulant or other autoimmune-related conditions. The test uses plasma from a blood donor sample.

Quick summary

["This test helps evaluate potential blood clotting issues or autoimmune disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 15, 2026Editorial policy

Key facts at a glance

LOINC
99785-8
Short code
cfDNA.DD%
Synonyms
cfDNA.donor/cfDNA.total p card trnsplt
Category
chemistry
Sample
plasma
Container
Streck Cell-Free DNA BCT tube (preferred) or EDTA plasma tube; avoid heparin tubes due to interference with downstream PCR/NGS assays
Units
N/A
Ref low
0
Ref high
0.7
Critical range
— – 1 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–99 years
Method
Next-generation sequencing (NGS) or targeted SNP-based quantification using digital PCR or shotgun sequencing; measurement of donor-derived cell-free DNA fraction via bioinformatic comparison to recipient genotyping
CPT
0118U (Plasma cell-free DNA quantification for allograft rejection, including donor-derived fraction; AlloSure Heart, CareDx)
SNOMED
122451002 |Measurement of cell-free DNA in plasma (procedure)|
Specimen volume
10 mL whole blood collected into Streck BCT tube; yields approximately 4–5 mL plasma after processing
Stability
Whole blood stable in Streck BCT tube: 7 days at 6–37°C; separated plasma: 24 hours at 2–8°C; long-term storage at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles (limit to 2 cycles).
Hemolysis max
100
Lipemia max
100
Icterus max
50
TAT
72–168 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test if you have symptoms like unusual bleeding, clotting problems, or signs of autoimmune diseases. It may also be used to monitor conditions like lupus or other connective tissue disorders.

What Does the Result Mean?

Results are not typically provided as a single numeric range because interpretation depends on clinical context, such as your symptoms, other test results, and medical history. Elevated levels may suggest conditions like lupus anticoagulant, while normal results do not rule out all possible causes.

How Is the Test Performed?

A blood sample is drawn from a vein in your arm. The plasma portion is tested for cardiolipin antibodies using an immunoassay method, often involving a donor plasma sample for comparison.

What Are Common Causes of Abnormal Results?

Abnormal results may be linked to autoimmune diseases (e.g., systemic lupus erythematosus), antiphospholipid syndrome, or other conditions affecting blood clotting. False positives can occur due to other medications or infections.

Reference range

Donor-derived cfDNA fraction (% donor cfDNA / total cfDNA) less than 0.5% (or <0.7% depending on assay) is generally considered negative/low risk for acute rejection in stable adult cardiac transplant recipients. Results are reported as a percentage. Reference values vary by laboratory and assay platform; refer to performing lab report.N/A

Specimen: plasma

No fasting required. Patient should be well-hydrated. Avoid strenuous exercise for at least 30 minutes prior to venipuncture, as physical activity may transiently elevate total cell-free DNA levels. Blood should be drawn prior to scheduled endomyocardial biopsy when possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

IgG Cardiolipin Antibody Total (Donor-Specific)

Frequently asked questions

What are the normal values for this test?
Normal values are not standardized in this context because interpretation depends on clinical context, such as your symptoms, other test results, and medical history. Your healthcare provider will discuss the meaning of your specific result with you.
How long does it take to get the results?
Results are typically available within 1–3 business days, depending on lab processing times. Follow-up with your provider to discuss the results and next steps.
Can I fast before this test?
No fasting is required for this blood test. However, always follow your provider’s instructions for best accuracy.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy