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Serum or Plasma Substance Concentration by Immunoassay (Bromocresol Green)

Published: August 9, 2026Updated: August 14, 2026

This test measures a specific substance in your blood using a bromocresol green-based immunoassay method. It helps detect or monitor certain proteins, enzymes, or other biomarkers.

Quick summary

["This test may assist in diagnosing or managing conditions affecting protein levels or specific metabolic pathways."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 14, 2026Editorial policy

Key facts at a glance

LOINC
7497-1
Short code
CHEESEMLDHR
Synonyms
Cheese Mold BasoBnd Ab Qn
Category
chemistry
Sample
whole-blood
Container
EDTA (purple-top) or heparin (green-top) blood collection tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
Basophil histamine release assay (flow cytometry or fluorometric method)
CPT
86003
SNOMED
428201007
Specimen volume
5-10 mL of whole blood
Stability
Specimen should be processed within 24 hours of collection when stored at room temperature (18-25°C). If testing is delayed, refrigerate at 2-8°C for up to 48 hours. Do not freeze whole blood specimens.
Hemolysis max
100
Lipemia max
100
Icterus max
50
TAT
48–168 hours
Order
routine

What This Test Measures

The test detects the concentration of a particular substance in your serum or plasma using a chemical reaction with bromocresol green. This method identifies the presence and amount of the target analyte through its interaction with antibodies or other binding proteins.

Why It’s Ordered

Your healthcare provider may order this test to evaluate conditions like liver disease, kidney function, infections, or certain genetic disorders. It can also help track treatment progress for conditions where specific biomarkers are relevant.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in the arm. The blood is processed to separate serum (if clotted) or plasma (if anticoagulated) for testing. This sample is then analyzed in a lab using the bromocresol green immunoassay technique.

Possible Results and Interpretation

Results are expressed as a concentration value, though exact reference ranges vary by analyte and lab. Your provider will interpret results in relation to your clinical context, such as symptoms, medical history, or other test results.

Reference range

Reference values vary by laboratory; results reported as histamine release in units per volume of blood. Negative or low response typically indicates no significant sensitivity. Interpretation should be performed by a qualified allergist in conjunction with clinical history.N/A

Specimen: whole-blood

No special patient preparation is typically required. Patients should continue their usual medications unless specifically instructed otherwise by their physician. Recent antihistamine use may affect basophil reactivity and should be documented. Patients should inform their healthcare provider of any recent allergic reactions or current allergy symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal results may indicate underlying health issues, such as abnormal protein synthesis, enzyme deficiencies, or systemic inflammatory processes. For example, elevated levels might suggest liver dysfunction, while low levels could point to genetic disorders or metabolic imbalances. Always discuss results with your healthcare provider for personalized insights.

Short name

BCG Immunoassay (Substance Concentration)

Frequently asked questions

What does this test measure?
This test measures the concentration of a specific substance in your blood using a specialized immunoassay method. The exact analyte depends on the clinical indication, but it often relates to proteins, enzymes, or other biomarkers relevant to your health condition.
How long will I need to prepare for this test?
No special preparation is typically required, but you may be asked to fast or avoid certain medications for 12 hours before the test, depending on the specific analyte being tested. Follow your healthcare provider’s instructions.
Can I take this test at home?
No, this test requires a blood sample collected by a healthcare professional in a clinical setting. Home blood tests for this specific method are not available.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 14, 2026. · Read our editorial policy