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High-Density Lipoprotein (HDL) Cholesterol, Serum/Plasma — Calculated

Published: August 9, 2026Updated: August 16, 2026

A calculated blood test that estimates the amount of cholesterol carried by high-density lipoprotein (HDL), sometimes called “good” cholesterol.

Quick summary

["HDL cholesterol helps assess cardiovascular risk, but it should be interpreted with your other cholesterol results and medical history."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
9322-9
Short code
Cholest/HDLc Prctl SerPl
Synonyms
Cholest/HDLc Prctl SerPl
Category
lipid
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
40
Ref high
60
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83700
SNOMED
113079008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test estimates HDL cholesterol in a blood sample. HDL is a protein-and-fat particle that carries cholesterol through the bloodstream. The result is usually calculated from other cholesterol measurements rather than measured directly.

Why might I need this test?

Your clinician may order it as part of a cholesterol panel to help assess your risk of heart disease and stroke. It may also be used to monitor lipid-lowering treatment or changes in your cardiovascular risk.

How is the test performed?

A blood sample is collected from a vein and analyzed in a laboratory. The laboratory calculates the HDL cholesterol value using measurements from the same or a related blood sample.

How do I prepare for the test?

You may be asked to fast for about 9 to 12 hours, depending on the laboratory and the other tests being ordered. Water is generally encouraged, and you should follow your clinician’s specific instructions.

Reference range

Adult males: >40 mg/dL; Females: >50 mg/dLN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Calculated HDL Cholesterol

Frequently asked questions

Is this the same as a direct HDL cholesterol test?
No. This result is usually calculated from other cholesterol measurements. A direct HDL test measures HDL cholesterol separately, so the two methods may produce slightly different results.
Does a high HDL cholesterol result eliminate heart disease risk?
No. A high HDL result is generally favorable, but it does not cancel out other cardiovascular risk factors or make preventive care unnecessary.
Do I need to fast before this test?
It depends on the test panel. Fasting may be required for accurate triglyceride measurement, which can also affect the calculated HDL value.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy