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High-Density Lipoprotein Cholesterol (HDL-C), Serum/Plasma

Published: August 9, 2026Updated: August 16, 2026

Measures HDL cholesterol in blood. HDL helps transport cholesterol away from artery walls to the liver, so its level is considered when assessing cardiovascular risk.

Quick summary

["HDL cholesterol is one part of a lipid panel used to help assess heart and blood vessel risk."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
9830-1
Short code
Cholest/HDLc SerPl
Synonyms
Cholest/HDLc SerPl
Category
lipid
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
40
Ref high
60
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83700
SNOMED
113079008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of cholesterol carried by high-density lipoprotein (HDL) in your blood. HDL is often called “good cholesterol” because it helps move cholesterol from tissues back to the liver.

Why might I need this test?

HDL cholesterol is usually measured as part of a lipid panel, which also includes total cholesterol, LDL cholesterol, and triglycerides. Your clinician may use the overall results to help assess your risk of heart attack, stroke, and other cardiovascular problems.

How is the test performed?

A blood sample is drawn from a vein and analyzed by a laboratory. The laboratory separates HDL from the other lipoproteins in the sample and measures the amount of cholesterol it contains.

How do I prepare for the test?

Follow your clinician’s instructions about fasting. Fasting for 9 to 12 hours may be required if this test is part of a complete lipid panel, and water is generally the only permitted drink. Tell your clinician if you are pregnant, have recently had surgery, or are taking a medication that could affect the result.

Reference range

Adult males: >40 mg/dL; Females: >50 mg/dLN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HDL Cholesterol

Frequently asked questions

Is a higher HDL cholesterol result always better?
Higher HDL levels are generally associated with lower cardiovascular risk, but very high levels do not necessarily mean that medication or additional treatment is needed. Your clinician will interpret the result with your other cholesterol measurements and risk factors.
Does HDL cholesterol show whether I have a heart disease?
No. This test helps estimate cardiovascular risk but does not diagnose heart disease. Symptoms, medical history, blood pressure, smoking status, and other tests are also important.
Can lifestyle changes affect HDL cholesterol?
Regular physical activity, a balanced diet, maintaining a healthy weight, and avoiding tobacco may help improve HDL cholesterol. Changes can vary among individuals, and your clinician can advise which steps are appropriate for you.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy