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24-Hour Urinary Calcium (Substance Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of calcium excreted in your urine over a 24-hour period. It helps assess kidney function and calcium balance in your body.

Quick summary

["Helps diagnose kidney issues or calcium-related disorders by tracking long-term urine calcium levels."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25876-4
Short code
Citrate 24h Ur-sCnc
Synonyms
Citrate 24h Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Matters

Your kidneys normally filter calcium from your blood and release it into your urine. If your body retains too much calcium (hypercalcemia) or if your kidneys can’t filter it properly, this test can reveal imbalances that may indicate conditions like kidney stones, kidney disease, or other metabolic disorders.

When You Might Need It

Your doctor may order this test if you have symptoms like frequent kidney stones, unexplained bone pain, high blood calcium levels, or if you’re at risk for kidney disease. It’s also used to monitor certain kidney-related conditions or metabolic disorders.

What to Expect Before the Test

You’ll need to collect all urine for 24 hours, starting right after you wake up and ending just before you wake up the next morning. Store the urine in a refrigerator or cool container, and keep a record of all fluids you drink. Your doctor or lab technician will provide detailed instructions.

What Happens During the Test

No special preparation is needed beyond collecting your urine sample. The lab will analyze the total calcium content in your urine over the full 24-hour period.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: urine

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24h Urine Calcium

Frequently asked questions

What causes abnormal urinary calcium levels?
Abnormal results may be linked to conditions like kidney stones, hyperparathyroidism, excessive vitamin D intake, or certain medications. Your doctor will consider your symptoms, medical history, and other test results to determine the cause.
How often should I get this test?
Your doctor will determine the frequency based on your health needs. Some patients may need it yearly or more often if they have ongoing kidney or metabolic issues.
Can I drink water during the 24-hour urine collection?
Yes, but you must account for all fluids you drink in your total urine volume. Avoid excessive caffeine or alcohol, as they can affect results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy