Serum or plasma protein electrophoresis with bromocresol green and bromocresol purple staining

What Proteins Does This Test Measure?

Electrophoresis separates blood proteins based on size and charge, revealing distinct bands. Bromocresol green and purple dyes highlight specific protein groups, including albumin, alpha-globulins, beta-globulins, and gamma-globulins. Abnormal patterns may indicate conditions like multiple myeloma, nephrotic syndrome, or chronic inflammation.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like unexplained weight loss, fatigue, swelling, or if they suspect conditions like kidney disease, liver disease, or autoimmune disorders. It’s also used to monitor treatment progress for certain cancers or protein-related disorders.

How Is the Test Performed?

A blood sample is drawn, and the serum or plasma is placed in a gel or paper strip. An electric current runs through the sample, causing proteins to migrate toward opposite charges. Dyes bind to specific proteins, creating visible bands that are analyzed.

Sample Preparation and Timing

Blood is typically collected in the morning for consistency. No special preparation is needed, but fasting may not be required unless your doctor specifies. Results may take several days to analyze, depending on lab volume.