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Lymphocyte Subpopulation Analysis by Flow Cytometry (Quantitative, Total Lymphocyte Count and Specific Subset Counts)

Published: August 9, 2026Updated: August 16, 2026

This test measures the types and amounts of white blood cells called lymphocytes in your blood, which help fight infections. It may be used to assess immune function or identify conditions affecting your immune system.

Quick summary

["Understanding your lymphocyte counts can help diagnose or monitor immune-related health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
74750-1
Short code
Cleft Lymphs Bld/T Ql Micro
Synonyms
Cleft Lymphs Bld/T Ql Micro
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
microscopy-light
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order this test to check for immune system disorders, such as infections, autoimmune diseases, or conditions like leukemia or lymphoma. It can also be used before or after treatments that affect the immune system, like chemotherapy or organ transplants.

What to Expect Before the Test

No special preparation is usually needed. You may be asked to fast for 8–12 hours before the blood draw if your test includes fasting components, but this is not always the case for lymphocyte subset analysis alone. Bring your medical records or questions to your appointment.

How the Test Is Performed

A healthcare professional will draw blood from a vein in your arm using a small needle. The blood sample is then analyzed in a lab to count and identify different types of lymphocytes, such as T-cells, B-cells, and natural killer cells.

What the Results Mean

Results show the total number of lymphocytes and the proportions of each subset. Abnormal counts may indicate immune deficiencies, overactive immune responses, or other health issues. Your doctor will interpret the results in the context of your overall health and symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Changes in lymphocyte counts can signal underlying conditions, such as weakened immunity (e.g., HIV/AIDS), overactive immune responses (e.g., rheumatoid arthritis), or cancers affecting the blood or bone marrow. Regular monitoring may be needed if you have chronic illnesses or undergo treatments that impact immune function.

Short name

Lymphocyte Profile

Frequently asked questions

How long does it take to get my results?
Results are typically available within 1–3 business days, depending on lab processing times. Your doctor will contact you once they are ready, or you may receive a summary in your electronic health record.
What does it feel like to have blood drawn?
Most people experience a brief sting or pinch when the needle is inserted. Afterward, you may feel a slight bruise or soreness at the site, which usually goes away within a few hours. Some people may also feel lightheaded or dizzy during the procedure.
Can I eat or drink before this test?
For standard lymphocyte subset analysis, no special fasting is required. However, if your doctor has specific instructions (e.g., fasting for glucose or other tests), follow those instead. Always confirm with your healthcare provider before the appointment.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy