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Carbon Dioxide in Blood (as Bicarbonate and Carbonic Acid)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of carbon dioxide in your blood, primarily in the form of bicarbonate, which helps regulate your body’s acid-base balance.

Quick summary

["Your blood’s CO₂ levels help maintain healthy pH and support normal breathing and kidney function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
14640-7
Short code
CO2 Dial fld-sCnc
Synonyms
CO2 Dial fld-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
22
Ref high
28
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82374
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Blood gases, including CO₂, are critical for keeping your body’s chemistry stable. Your blood carries carbon dioxide from tissues to the lungs, where it’s exhaled. This test checks the total CO₂ content, which includes bicarbonate (a key buffer) and carbonic acid (formed when CO₂ dissolves in blood).

Why It Matters in Your Health

Abnormal CO₂ levels can signal conditions like respiratory issues (e.g., asthma, COPD), metabolic problems (e.g., kidney disease, diabetes), or fluid imbalances. High levels may indicate overproduction of CO₂ (e.g., hyperventilation), while low levels could suggest breathing difficulties or metabolic acidosis.

Common Conditions Affecting Results

This test is often ordered when you have symptoms like shortness of breath, chest pain, confusion, or rapid heartbeat. It may also be used to monitor chronic conditions like heart failure, kidney disease, or severe infections.

Sample Collection and Preparation

A blood sample is taken from a vein (usually in your arm) and sent to a lab for immediate analysis. No special preparation is needed, but fasting may be required depending on the clinical context.

Reference range

Adult: 22-28 mmol/LN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

CO₂ (blood gas)

Frequently asked questions

How does CO₂ testing help doctors diagnose health issues?
CO₂ testing helps identify acid-base imbalances (e.g., respiratory acidosis or alkalosis) and assesses lung function, kidney function, or metabolic disorders. It’s especially useful in emergency settings or for patients with chronic lung or heart conditions.
Can diet or medications affect this test?
Certain foods (e.g., high-protein or high-fat meals) or medications (e.g., diuretics, asthma inhalers) may influence blood gas results. Your doctor will clarify any dietary or medication restrictions before testing.
What if my results are outside the normal range?
If your CO₂ levels are abnormal, your doctor will review your symptoms and other test results to determine the cause. Treatment may include breathing exercises, medication adjustments, or further medical evaluation.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy