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Cortisol (Salivary, Free, 15-Minute Free Cortisol)

Published: August 9, 2026Updated: August 16, 2026

This test measures cortisol levels in your saliva over a 15-minute period to assess how your body responds to stress or hormonal signals.

Quick summary

Helps evaluate adrenal function and stress response by tracking cortisol release in saliva.

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58650-3
Short code
Cortis 15M pre 1 ug/kg CRH IV Sal-mCnc
Synonyms
Cortis 15M pre 1 ug/kg CRH IV Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
86701
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect conditions like Cushing’s syndrome, adrenal insufficiency, or abnormal cortisol rhythms (e.g., due to stress, medications, or pituitary disorders). It can also help diagnose or monitor conditions affecting cortisol production or metabolism.

What to Expect Before the Test

No special preparation is needed. Collect saliva samples at specific times (e.g., morning or during stress events) using a provided kit. Follow instructions carefully to ensure accurate results.

How the Test Works

Saliva is collected in a sterile container at designated intervals. The cortisol in your saliva is measured using a sensitive immunoassay method to detect its presence and concentration.

What the Results Mean

Results help determine whether cortisol levels are too high or too low at specific times. Abnormal findings may indicate hormonal imbalances or other health concerns requiring further evaluation.

Frequently asked questions

Why might my doctor recommend this test?
Your doctor may recommend this test if they suspect hormonal issues affecting cortisol, such as Cushing’s syndrome (too much cortisol), adrenal fatigue, or problems with your pituitary gland (which regulates cortisol). It can also help monitor stress-related cortisol fluctuations or evaluate responses to treatments.
What are typical reference ranges for this test?
Reference ranges vary by lab and testing protocol. Your results will be compared to your specific lab’s standards, which consider factors like time of day, collection method, and individual health. Ask your doctor for a personalized interpretation of your results.
Can I take medications before this test?
Some medications (e.g., steroids, blood pressure drugs) may affect cortisol levels. Follow your doctor’s instructions about timing or stopping medications before collection. Avoid caffeine or stress-inducing activities during the test period to minimize interference.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy