Cortisol concentration in saliva (24-hour average)

Why This Test Matters

Cortisol is a key hormone produced by your adrenal glands, which sit on top of your kidneys. It plays a vital role in managing stress, immune response, and metabolism. Chronic stress or certain medical conditions can disrupt cortisol levels, leading to health issues like fatigue, weight changes, or hormonal imbalances. Salivary cortisol testing is less invasive than blood tests and can provide a more accurate picture of your daily hormone activity.

What to Expect Before the Test

No special preparation is needed for this test. You’ll collect saliva samples throughout the day, typically in a kit provided by your healthcare provider. Simply spit into the collection tubes at designated times (often every few hours). Return the samples to your lab or clinic as instructed. Avoid brushing your teeth before collecting samples to prevent interference.

What Happens During the Test

The test involves collecting saliva samples in a sterile container. You’ll likely be given a kit with multiple tubes and instructions on when to collect each sample. Some kits may require you to return the samples to a lab or clinic within a specific timeframe. The samples are then analyzed to measure cortisol levels over the 24-hour period.

What the Results Mean

Your results will show the average cortisol concentration in your saliva over 24 hours. While specific reference ranges can vary by lab, abnormal results may indicate either too much cortisol (hypercortisolemia) or too little (hypocortisolemia). Your healthcare provider will interpret the results in the context of your symptoms and medical history to determine if further testing or treatment is needed.