Cortisol with 3-hour triple bolus (serum/plasma) measurement using substance concentration method

Why This Test Is Done

Your doctor may recommend this test if you have symptoms like fatigue, weight changes, salt cravings, or unexplained stress responses. It’s often used in patients suspected of having conditions like Cushing’s syndrome or adrenal insufficiency, where cortisol levels fluctuate abnormally.

What to Expect Before the Test

You’ll likely receive a dose of a synthetic hormone (like ACTH) to stimulate cortisol production. Blood samples will be taken at three different times: before the dose, and 30 minutes and 60 minutes after administration. Avoid caffeine or alcohol before testing as it may affect results.

What Happens During the Test

A healthcare provider will draw blood from a vein in your arm at each scheduled time. The blood will be sent to a lab for analysis. The test typically takes about 1 hour to complete, including preparation and sample collection.

What the Results Mean

The results help determine if your body’s cortisol production is functioning normally. Abnormally high or low cortisol levels can indicate underlying health issues that need medical attention.