Cortisol (Serum) – Mass Concentration
This test measures the amount of cortisol, a natural steroid hormone produced by your adrenal glands, in your blood serum. Cortisol helps regulate metabolism, immune response, and stress reactions.
["Cortisol levels help assess adrenal function and potential stress-related conditions."]
Key facts at a glance
- LOINC
- 1404-3
- Short code
- Cortis 1h p Ins IV SerPl-mCnc
- Synonyms
- Cortis 1h p Ins IV SerPl-mCnc
- Category
- hormone
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Might Be Ordered
Your healthcare provider may recommend this test if you have symptoms like unexplained weight changes, fatigue, muscle weakness, or irregular menstrual cycles. It could also be used to diagnose or monitor conditions such as Cushing’s syndrome, adrenal insufficiency, or stress-related hormone imbalances.
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if cortisol is measured at a specific time (e.g., morning or evening). If you’re taking medications that affect cortisol levels, inform your provider.
How the Test Is Performed
A blood sample is drawn from a vein, typically in your arm. The sample is then sent to a lab for analysis using a sensitive method to measure cortisol concentration.
What the Results Mean
Results are compared to standard reference ranges, but individual ranges can vary. High or low cortisol levels may indicate underlying health concerns that need further evaluation by a healthcare provider.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
High cortisol levels may suggest conditions like Cushing’s syndrome (overproduction of cortisol) or chronic stress. Low cortisol levels could indicate adrenal insufficiency (e.g., Addison’s disease) or other hormonal imbalances. Your provider will interpret results in the context of your symptoms and medical history.
Short name
Serum Cortisol
Frequently asked questions
What causes cortisol levels to be tested?
How long does it take to get results?
Can diet or medications affect cortisol test results?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy