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24-Hour Urine Free Cortisol

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, excreted in your urine over a 24-hour period. It helps assess how well your body produces and regulates cortisol levels.

Quick summary

["This test helps diagnose conditions related to cortisol imbalance, such as Cushing’s syndrome or adrenal insufficiency."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
83089-3
Short code
Cortis 24h Ur IA-mCnc
Synonyms
Cortis 24h Ur IA-mCnc
Category
hormone
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend a 24-hour urine free cortisol test if they suspect hormonal imbalances, such as overproduction of cortisol (Cushing’s syndrome) or adrenal gland issues. It’s particularly useful when blood cortisol tests alone don’t provide enough information.

How the Test Works

You’ll collect all your urine for a full 24-hour period, typically starting and ending at the same time each day. The collected urine is then sent to a lab for analysis. This method captures the total cortisol excreted in your body over a day, giving a more accurate picture than single blood tests.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to avoid certain medications or supplements that could affect results. Your doctor will provide specific instructions if needed.

What to Expect During the Test

You’ll need to collect every bit of urine produced during the 24-hour period, including during the night. Use a clean container or urine collection kit provided by your healthcare team. Make sure to record the time each sample is collected for accurate tracking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24h Free Cortisol

Frequently asked questions

What does a high or low result mean?
A high result may signal overproduction of cortisol, which can lead to symptoms like weight gain, high blood pressure, or muscle weakness. A low result might indicate problems with your adrenal glands or pituitary gland, which regulate cortisol. Your doctor will interpret results in the context of your symptoms and other tests.
How often should I get this test?
Your doctor will determine how often you need this test based on your symptoms, medical history, and other test results. It may be done once or repeated over time to monitor changes in your condition.
Can I take medications before this test?
Some medications, like steroids or certain antidepressants, can affect cortisol levels. Your doctor will advise you whether to stop or adjust these medications before the test or if they need to be accounted for in the results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy