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24-Hour Urine Collection for Cortisol (Substance Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, excreted in your urine over a 24-hour period. It helps assess your body’s cortisol production and adrenal gland function.

Quick summary

["This test helps diagnose conditions related to cortisol levels, such as Cushing’s syndrome or adrenal insufficiency."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50848-1
Short code
Cortis 24h Ur-sCnc
Synonyms
Cortis 24h Ur-sCnc
Category
hormone
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect conditions like Cushing’s syndrome (too much cortisol) or adrenal insufficiency (too little cortisol). It can also be used to monitor treatment effectiveness for hormone-related disorders.

How the Test Is Collected

You’ll need to collect all urine for a full 24-hour period, typically starting after midnight of one day and ending just before midnight the next. A healthcare provider will provide instructions on proper collection and storage to ensure accuracy.

What to Expect During the Test

You’ll likely need to wear a special collection container or use a urine collection kit provided by your healthcare team. Some patients may need to empty their bladder at the start and end of the collection period to ensure complete accuracy.

Preparation Tips

Follow your doctor’s instructions carefully. Avoid certain medications that may affect cortisol levels, and inform your doctor about any over-the-counter supplements or herbal products you’re taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated cortisol levels in urine may indicate Cushing’s syndrome, caused by prolonged exposure to high cortisol levels, often due to tumors or excess hormone production. Low cortisol levels can signal adrenal insufficiency, where the adrenal glands fail to produce enough cortisol, potentially due to autoimmune disorders or other medical conditions.

Short name

24h Urine Cortisol

Frequently asked questions

How long does it take to get results from this test?
Results are typically available within 1 to 2 weeks after the sample is processed, depending on your lab’s workflow and any follow-up testing required.
What does it mean if my cortisol levels are too high or too low?
High cortisol levels may suggest conditions like Cushing’s syndrome, which can cause weight gain, fatigue, or high blood pressure. Low cortisol levels might indicate adrenal insufficiency, leading to symptoms like dizziness, weakness, or low blood pressure, especially upon standing.
Can diet or lifestyle affect this test?
Yes, certain foods, medications, and even stress can influence cortisol levels. It’s important to follow your healthcare provider’s instructions regarding diet, hydration, and activity levels during your 24-hour urine collection to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy