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Cortisol, Free, 24-Hour Urinary Metabolite (Total Cortisol Metabolites)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of cortisol metabolites excreted in your urine over 24 hours. It helps assess your body’s cortisol production, often used to diagnose conditions like Cushing’s syndrome or adrenal insufficiency.

Quick summary

["Helps diagnose hormonal imbalances affecting stress response and cortisol levels."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59803-7
Short code
Cortis 30M p chal SerPl-SCDiff
Synonyms
Cortis 30M p chal SerPl-SCDiff
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend this test if they suspect an overactive or underactive adrenal gland, unusual cortisol levels, or conditions linked to chronic stress or hormone-related disorders. It’s also used to monitor treatment effectiveness for conditions like Cushing’s syndrome.

What to Expect Before the Test

You’ll collect your urine for 24 hours, starting and ending at the same time each day. Store the urine in a refrigerator or a cool, dark place until you deliver it to the lab. Follow your doctor’s instructions carefully to ensure accurate results.

During the Test

No special preparation is needed beyond collecting urine over 24 hours. Your sample will be analyzed in a lab to measure cortisol metabolites.

After the Test

You’ll receive your results from your healthcare provider. High levels may indicate conditions like Cushing’s syndrome, while low levels could suggest adrenal insufficiency or other hormonal issues.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urinary Cortisol Test

Frequently asked questions

What does a high result mean?
A high result may suggest conditions such as Cushing’s syndrome (excess cortisol), prolonged stress, or certain medications that increase cortisol. Your doctor will review your symptoms and other test results to determine the cause.
What does a low result mean?
A low result may indicate adrenal insufficiency (where the adrenal glands don’t produce enough cortisol), or conditions like Addison’s disease. This could also occur with certain medications or chronic illnesses.
How often should I get this test?
Your doctor will determine the frequency based on your symptoms, medical history, and treatment needs. Regular monitoring may be required if you have a known hormonal disorder or are on long-term medication affecting cortisol levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy