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Cortisol, 30-Minute Post-Rapid Corticotropin Stimulation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures your cortisol levels in blood after taking a hormone stimulant to check how your body responds to stress.

Quick summary

Helps diagnose adrenal gland issues or cortisol imbalance after a stress trigger.

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58662-8
Short code
Cortis 30M pre 1 ug/kg CRH IV Sal-sCnc
Synonyms
Cortis 30M pre 1 ug/kg CRH IV Sal-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
86701
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like fatigue, weight changes, or unusual mood swings that could suggest adrenal problems. It’s also used to confirm conditions like Cushing’s syndrome or adrenal insufficiency.

What to Expect Before the Test

You’ll receive a small injection of corticotropin (ACTH), a hormone that stimulates cortisol production. Blood is drawn 30 minutes later to check cortisol levels. Avoid caffeine or stress before testing.

What Happens During the Test

A healthcare provider will draw blood from a vein in your arm. You may feel slight discomfort, but it’s quick. The test checks cortisol concentration in your serum.

What the Results Mean

Abnormal results may indicate problems with your adrenal glands or how your body handles stress. Your doctor will compare your results to your baseline and other tests.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Cortisol plays a key role in managing stress, metabolism, and immune response. Low cortisol may signal adrenal insufficiency, while high levels could suggest overproduction (e.g., Cushing’s). This test helps diagnose these conditions by assessing cortisol response to ACTH stimulation.

Short name

Cortisol Stimulation Test

Frequently asked questions

What causes abnormal cortisol levels in this test?
Possible causes include adrenal gland disorders (e.g., Addison’s disease), Cushing’s syndrome, or pituitary issues. Other factors like medications, stress, or recent illness can also affect results.
How long does it take to get results?
Results are typically available within 1–3 business days, depending on lab processing. Your doctor will discuss them with you during a follow-up visit.
Can I eat or drink before this test?
Standard fasting rules may apply, but check with your doctor. Avoid caffeine or alcohol for 24 hours before testing to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy