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Cortisol (Serum) with Stimulation by CRH (Corticotropin-Releasing Hormone)

Published: August 9, 2026Updated: August 16, 2026

This test measures your cortisol levels after receiving a hormone trigger to help assess how your body responds to stress and regulate cortisol production.

Quick summary

This test helps diagnose conditions affecting cortisol production or stress responses.

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58663-6
Short code
Cortis 30M pre 1 ug/kg CRH IV SerPl-mCnc
Synonyms
Cortis 30M pre 1 ug/kg CRH IV SerPl-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
86701
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect conditions like Cushing’s syndrome (too much cortisol), adrenal insufficiency (not enough cortisol), or pituitary disorders. It can also evaluate how your body reacts to stress or certain medications.

What to Expect Before the Test

You’ll receive an injection of CRH, a hormone that stimulates cortisol release. Blood will be drawn before and after the injection to measure cortisol levels. Avoid caffeine or stressing activities before the test.

How the Test Works

CRH is injected to trigger cortisol production. Blood samples are taken at baseline and after 30 minutes to measure cortisol concentration. This helps determine if your adrenal glands respond normally to stress signals.

What the Results Mean

Results compare baseline cortisol to the stimulated level. Abnormal results may indicate issues with cortisol production, stress tolerance, or hormone imbalances that need further medical evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Cortisol and Stress Response

Short name

CRH-Stimulated Cortisol

Frequently asked questions

What does cortisol do in my body?
Cortisol is a hormone produced by your adrenal glands that helps manage stress, regulate metabolism, and support immune function. It peaks in the morning and lowers at night. Abnormal levels can signal conditions like adrenal disorders or Cushing’s syndrome.
Why is CRH used in this test?
CRH is used to stimulate cortisol production because it mimics natural stress signals. This helps doctors assess whether your adrenal glands can respond appropriately to stress, which may not be clear from a single cortisol measurement alone.
Can I take medications before this test?
Yes, your doctor may ask you to stop certain medications (like steroids) before the test. Follow their instructions carefully to get accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy