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Cortisol, 30-Minute Post-Resting Stimulation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures your cortisol levels in serum after a short period of rest, followed by a controlled stimulation to assess how your body responds to stress hormones.

Quick summary

["Helps evaluate adrenal function and stress response over time."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50441-5
Short code
Cortis 30M pre Ins IV SerPl-mCnc
Synonyms
Cortis 30M pre Ins IV SerPl-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is This Test For?

Doctors may order this test if they suspect conditions like adrenal insufficiency, Cushing’s syndrome, or other hormonal imbalances that affect cortisol production. It can also help diagnose whether your body’s stress response is functioning normally after a period of rest.

How Is the Test Performed?

You’ll have a blood sample drawn from a vein after resting quietly for about 30 minutes. The sample is then sent to a lab for analysis using an immunoassay method to measure cortisol concentration.

What to Expect Before the Test

No special preparation is needed, but avoid caffeine or stress-inducing activities before the test. Wear comfortable clothing and plan to stay for the blood draw.

Common Conditions This Test Helps Diagnose

This test may help identify issues such as abnormal cortisol secretion, whether from overproduction (e.g., Cushing’s syndrome) or underproduction (e.g., adrenal insufficiency), or assess how your body reacts to stress hormones.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Cortisol is a critical hormone produced by the adrenal glands, helping regulate metabolism, immune response, and stress reactions. Abnormal levels can indicate underlying health concerns requiring medical evaluation.

Short name

Cortisol Response Test

Frequently asked questions

Why might my doctor recommend this test?
Your doctor may suggest this test if they suspect hormonal imbalances, stress-related conditions, or adrenal gland dysfunction based on symptoms like fatigue, weight changes, or mood swings.
How long will I need to wait for results?
Results are typically available within 1–3 business days, depending on lab processing times. Your doctor will discuss them with you during a follow-up visit.
Can I eat or drink before this test?
Yes, you can eat and drink normally before the test, but avoid caffeine or alcohol for at least 24 hours prior, as they may affect cortisol levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy