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Cortisol, 24-hour urinary free cortisol (mass concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, excreted in your urine over a 24-hour period. It helps assess how much cortisol your body produces and whether it’s working properly.

Quick summary

["This test helps diagnose conditions related to cortisol levels, such as Cushing’s syndrome or adrenal insufficiency."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
54212-6
Short code
Cortis 3D p Dex SerPl-sCnc
Synonyms
Cortis 3D p Dex SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend this test if they suspect hormonal imbalances, such as an overactive or underactive adrenal gland, or if you have symptoms like unexplained weight gain, fatigue, or unusual mood changes that might be linked to cortisol levels.

How the Test Works

You’ll collect all urine for a full 24 hours, typically starting after you wake up and ending just before you wake up the next day. The sample is then analyzed to measure cortisol concentration.

Sample Collection

To ensure accurate results, it’s important to follow your healthcare provider’s instructions precisely. You’ll need to keep a urine log, store samples at room temperature, and avoid certain foods or medications that could affect cortisol levels.

What to Expect Before the Test

No special preparation is usually needed, but you may be advised to avoid caffeine, alcohol, or certain medications for a few days before testing to reduce potential interference.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated levels may indicate Cushing’s syndrome (too much cortisol) or other conditions like hyperthyroidism. Low levels could suggest adrenal insufficiency or conditions like Addison’s disease. This test helps guide treatment decisions for hormonal disorders.

Short name

24-Hour Urine Cortisol

Frequently asked questions

What does a high or low result mean?
A high result might suggest conditions like Cushing’s syndrome, where the body produces too much cortisol, or certain tumors affecting hormone production. A low result could indicate adrenal gland problems or other hormonal imbalances.
How long will I need to wait for results?
Results are typically available within 1 to 2 weeks, depending on your lab’s processing time. Your doctor will discuss them with you during a follow-up visit.
Can I take medications before this test?
Some medications, like certain steroids or blood pressure drugs, may affect cortisol levels. Always check with your doctor or lab technician before taking any medications on the day of collection or before starting the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy