Cortisol (Substance Concentration) in Saliva
This test measures the amount of cortisol, a natural steroid hormone, present in your saliva. It helps assess your body’s stress response and cortisol levels over time.
["Saliva cortisol testing can help evaluate stress-related hormone balance and potential adrenal function issues."]
Key facts at a glance
- LOINC
- 58667-7
- Short code
- Cortis 4 AM specimen Sal-sCnc
- Synonyms
- Cortis 4 AM specimen Sal-sCnc
- Category
- hormone
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Saliva cortisol testing is often used to diagnose conditions like Cushing’s syndrome (overactive adrenal glands) or Addison’s disease (underactive adrenal glands). It may also be used to monitor stress levels or evaluate pituitary-adrenal axis function in people with chronic stress, depression, or other hormonal disorders.
How the Test Works
A small saliva sample is collected, typically over 24 hours or at specific times (morning and evening). The test measures cortisol levels in the saliva, which reflects your body’s natural cortisol production throughout the day.
Sample Collection and Preparation
You’ll provide a saliva sample using a provided collection device. Follow instructions carefully to ensure accurate results. Avoid eating, drinking, or brushing your teeth for at least 30 minutes before collecting the sample.
What to Expect Before the Test
No special preparation is usually needed, but inform your healthcare provider if you’re taking medications that could affect cortisol levels (e.g., steroids). Avoid caffeine or alcohol before collection if instructed.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Saliva cortisol levels can indicate whether your body is producing too much or too little cortisol. Abnormal results may signal conditions affecting your adrenal glands, pituitary gland, or stress response. Repeated testing may be needed to confirm diagnoses or monitor treatment progress.
Short name
Saliva Cortisol
Frequently asked questions
How long does it take to get results?
What does a high cortisol level mean?
Can I take medications before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy