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Cortisol, Serum (8 AM)

Published: August 9, 2026Updated: August 16, 2026

This test measures your cortisol hormone level in the morning to help assess your body’s ability to produce cortisol, which is important for managing stress, immune function, and other vital processes.

Quick summary

["Morning cortisol helps diagnose adrenal gland issues or stress-related hormone imbalances."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
45050-2
Short code
Cortis 8 AM SerPl-sCnc
Synonyms
Cortis 8 AM SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like fatigue, weight changes, salt cravings, or unexplained low blood pressure. It can also be used to evaluate conditions like Cushing’s syndrome, adrenal insufficiency, or stress-related hormone imbalances.

What to Expect Before the Test

No special preparation is needed, but you should fast for 8–12 hours before the test, starting from midnight the night before. Bring your test results and any medications you’re currently taking to your appointment.

How the Test Is Performed

A blood sample is drawn from a vein in your arm by a healthcare professional. You may feel a small pinch when the needle is inserted, but the procedure usually takes less than 5 minutes.

What the Results Mean

Results are compared to standard reference ranges for cortisol levels at 8 AM. Low levels may indicate adrenal insufficiency, while high levels could suggest overproduction of cortisol or other conditions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Morning cortisol level

Frequently asked questions

What causes abnormal cortisol levels?
Conditions like Cushing’s syndrome (too much cortisol), Addison’s disease (too little cortisol), or chronic stress can affect cortisol levels. Other factors, such as certain medications or thyroid disorders, may also influence results.
How long does it take to get results?
Results are typically available within 1–3 business days, depending on your lab and healthcare provider. Follow-up may be needed if your doctor suspects a condition requiring further testing.
Can I take medications before the test?
Yes, but inform your doctor or lab technician about all medications, supplements, or herbal products you’re taking. Some may affect cortisol levels, so they should be noted for accuracy.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy