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Cortisol (Salivary, Free)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, present in your saliva. Cortisol helps regulate metabolism, immune response, and stress reactions.

Quick summary

["Salivary cortisol reflects your body’s natural cortisol levels over time, useful for assessing stress and hormone balance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58676-8
Short code
Cortis 8 PM Sal-mCnc
Synonyms
Cortis 8 PM Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Salivary cortisol testing is often used to evaluate chronic stress, adrenal function, or conditions like Cushing’s syndrome or Addison’s disease. Unlike blood tests, saliva samples are easier to collect and provide a more natural representation of cortisol levels throughout the day.

What to Expect Before the Test

No special preparation is needed. Collect a saliva sample at home or during a clinic visit—usually in the morning for the most accurate results, as cortisol levels peak early in the day.

How the Test Works

A small amount of saliva is collected in a container. The sample is then analyzed to measure free cortisol levels, which are not bound to proteins in your body.

What the Results Mean

Results help doctors diagnose hormonal imbalances or stress-related conditions. High levels may indicate Cushing’s syndrome or excessive stress, while low levels could signal adrenal insufficiency.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Salivary Free Cortisol

Frequently asked questions

Why might my doctor order this test?
Your doctor may recommend this test if they suspect conditions like Cushing’s syndrome, Addison’s disease, or chronic stress affecting your hormones. It’s also used to monitor treatment for these conditions.
How often should I expect this test?
The frequency depends on your medical needs. Some patients may need it once, while others may require follow-up tests over months or years to track changes in cortisol levels.
What if my results are outside the normal range?
If your results are abnormal, your doctor will discuss next steps, which may include additional tests, lifestyle changes, or treatment adjustments to address the underlying cause.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy