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Cortisol, Free, Saliva

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, present in your saliva. It helps assess your body’s stress response and adrenal function.

Quick summary

["Saliva cortisol testing can help diagnose conditions linked to stress or adrenal gland issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58677-6
Short code
Cortis 8 PM Sal-sCnc
Synonyms
Cortis 8 PM Sal-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend this test if you have symptoms like fatigue, weight changes, or unexplained stress. Saliva cortisol is often used to check for conditions like Cushing’s syndrome or Addison’s disease, where hormone levels fluctuate throughout the day.

How the Test Works

A small saliva sample is collected, usually in the morning, to measure cortisol levels. Unlike blood tests, saliva cortisol reflects recent hormone activity without interference from other substances in the blood.

What to Expect

You’ll likely collect the sample at home using a provided kit, or it may be done in a lab. Results help determine if your cortisol levels are too high or too low over time.

Possible Results and Next Steps

Results are compared to typical ranges for your age and health. Abnormal levels may prompt further testing, like blood cortisol or a 24-hour urine test, to confirm a diagnosis.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Cortisol plays a key role in managing stress, immune response, and metabolism. Low cortisol can indicate adrenal insufficiency, while high levels may signal conditions like Cushing’s syndrome or excessive stress.

Short name

Free Saliva Cortisol

Frequently asked questions

What does a high or low saliva cortisol level mean?
High levels may suggest overproduction of cortisol (e.g., from a tumor or prolonged stress), while low levels could indicate adrenal gland problems or other hormonal imbalances. Your doctor will interpret results in the context of your symptoms and medical history.
Can diet or medications affect saliva cortisol results?
Yes, certain foods, supplements, or medications (like steroids) can influence cortisol levels. Your doctor may ask you to avoid caffeine, alcohol, or certain medications before testing for more accurate results.
How often should I get this test?
Frequency depends on your symptoms and diagnosis. If you’re monitoring a known condition, your doctor may recommend periodic testing, such as morning saliva samples over several days, to track trends.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy