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Cortisol, Serum (8 AM)

Published: August 9, 2026Updated: August 16, 2026

This test measures your cortisol hormone level in your blood at 8 AM to help assess your body’s ability to produce cortisol, which is important for managing stress, immune function, and other vital processes.

Quick summary

["Helps diagnose conditions like adrenal insufficiency or Cushing’s syndrome by checking cortisol levels at a specific time of day."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
48105-1
Short code
Cortis 8 PM SerPl-mCnc
Synonyms
Cortis 8 PM SerPl-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Cortisol is a hormone produced by your adrenal glands, which sit on top of your kidneys. It plays a key role in how your body responds to stress, regulates metabolism, and supports immune function. Measuring cortisol at 8 AM helps doctors evaluate whether your body is producing enough cortisol or if there’s an imbalance.

What to Expect Before the Test

No special preparation is needed for this test. You’ll have a small needle inserted into your arm to draw a blood sample. It usually takes a few minutes. If you’re taking medications that affect cortisol levels, let your healthcare provider know before the test.

What the Results Mean

Results help determine if your cortisol levels are too high or too low. Low cortisol levels (adrenal insufficiency) may cause fatigue, dizziness, or low blood pressure. High cortisol levels (Cushing’s syndrome) might lead to weight gain, high blood pressure, or muscle weakness. Your doctor will interpret the results in the context of your overall health.

Common Conditions This Test Helps Diagnose

This test is often used to diagnose conditions like Addison’s disease (adrenal insufficiency), Cushing’s syndrome (excess cortisol), or to check for problems with the pituitary gland, which controls cortisol production.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Morning cortisol level

Frequently asked questions

What causes abnormal cortisol levels?
Abnormal cortisol levels can be caused by a variety of factors, including chronic stress, certain medications (like steroids), thyroid disorders, or adrenal gland issues. If you’ve been experiencing symptoms like unexplained weight changes, fatigue, or mood swings, your doctor may recommend this test to investigate.
How often should I get this test?
The frequency of this test depends on your symptoms and medical history. Your doctor will determine if you need retesting, especially if you’re being treated for a condition related to cortisol levels, such as Cushing’s syndrome or Addison’s disease.
Does fasting affect this test?
No, fasting is not required for this test. Cortisol levels can naturally vary throughout the day, so measuring them at 8 AM provides a consistent reference point for comparison.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy