Cortisol, Serum (8 AM)
This test measures your cortisol hormone level in your blood at 8 AM to help assess your body’s ability to produce cortisol, which is important for managing stress, immune function, and other vital processes.
["Helps diagnose conditions like adrenal insufficiency or Cushing’s syndrome by checking cortisol levels at a specific time of day."]
Key facts at a glance
- LOINC
- 48105-1
- Short code
- Cortis 8 PM SerPl-mCnc
- Synonyms
- Cortis 8 PM SerPl-mCnc
- Category
- hormone
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Matters
Cortisol is a hormone produced by your adrenal glands, which sit on top of your kidneys. It plays a key role in how your body responds to stress, regulates metabolism, and supports immune function. Measuring cortisol at 8 AM helps doctors evaluate whether your body is producing enough cortisol or if there’s an imbalance.
What to Expect Before the Test
No special preparation is needed for this test. You’ll have a small needle inserted into your arm to draw a blood sample. It usually takes a few minutes. If you’re taking medications that affect cortisol levels, let your healthcare provider know before the test.
What the Results Mean
Results help determine if your cortisol levels are too high or too low. Low cortisol levels (adrenal insufficiency) may cause fatigue, dizziness, or low blood pressure. High cortisol levels (Cushing’s syndrome) might lead to weight gain, high blood pressure, or muscle weakness. Your doctor will interpret the results in the context of your overall health.
Common Conditions This Test Helps Diagnose
This test is often used to diagnose conditions like Addison’s disease (adrenal insufficiency), Cushing’s syndrome (excess cortisol), or to check for problems with the pituitary gland, which controls cortisol production.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Morning cortisol level
Frequently asked questions
What causes abnormal cortisol levels?
How often should I get this test?
Does fasting affect this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy