Cortisol in Cerebrospinal Fluid (Mass Concentration)
This test measures the amount of cortisol, a natural steroid hormone, in your cerebrospinal fluid (CSF). It helps assess how your brain and adrenal glands are producing and regulating cortisol, which plays a role in stress response and other bodily functions.
["This test may help diagnose conditions affecting cortisol levels in the brain or spinal fluid."]
Key facts at a glance
- LOINC
- 33257-7
- Short code
- Cortis CSF-mCnc
- Synonyms
- Cortis CSF-mCnc
- Category
- hormone
- Sample
- csf
- Container
- Sterile tube
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 1-2 mL
- Stability
- Immediate analysis required, Refrigerated: 1h
- Hemolysis max
- 200
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your healthcare provider may order this test if you have symptoms like severe headaches, nausea, vomiting, or neurological issues that could suggest conditions such as Cushing’s syndrome (excess cortisol), adrenal insufficiency, or pituitary disorders. It may also be used in cases of suspected brain tumors or other central nervous system disorders affecting hormone production.
What to Expect Before the Test
No special preparation is typically needed. You may have a lumbar puncture (spinal tap) performed to collect CSF. This procedure involves inserting a needle into your lower back to draw fluid. You may feel mild pressure or discomfort, but it usually doesn’t last long.
What the Test Results Mean
Cortisol levels in CSF can vary based on the time of day, stress levels, and underlying health conditions. High levels might indicate certain brain or pituitary disorders, while very low levels could suggest adrenal or pituitary problems. Your results will be interpreted by a healthcare provider in the context of your symptoms and medical history.
Common Conditions This Test Helps Diagnose
This test is often used to diagnose or evaluate conditions like Cushing’s disease (due to pituitary tumors), adrenal insufficiency, or other central nervous system disorders affecting hormone regulation. It may also be part of a broader evaluation for suspected brain tumors or other neurological issues linked to abnormal hormone production.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance: Cortisol in CSF plays a critical role in identifying disorders where the brain or pituitary gland improperly regulates cortisol. Abnormal levels can signal underlying conditions that require medical attention, such as tumors, infections, or hormonal imbalances. Early detection can guide treatment decisions.
Short name
CSF Cortisol
Frequently asked questions
What does a cortisol test in CSF measure?
How is the CSF collected for this test?
Can cortisol levels in CSF change based on when the test is done?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy