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Cortisol, Urine Free Metabolite (Total 16-Hydroxycortisol)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol metabolites excreted in your urine over a 24-hour period. It helps assess your body’s cortisol production and adrenal gland function.

Quick summary

["This test helps diagnose conditions related to cortisol imbalance, such as Cushing’s syndrome or adrenal insufficiency."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
111471-9
Short code
Cortis F Msmt Ur
Synonyms
Cortis F Msmt Ur
Category
hormone
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend this test if you have symptoms like unexplained weight gain, high blood pressure, muscle weakness, or unusual fatigue. It can also be used to monitor conditions affecting cortisol levels, such as Cushing’s syndrome or adrenal disorders.

What to Expect Before the Test

You’ll need to collect a 24-hour urine sample starting from one point in the morning and ending the next morning. Follow your doctor’s instructions carefully to ensure accurate results. Drink plenty of fluids but avoid caffeine or certain medications that could affect the test.

How the Test Works

Your urine is collected and analyzed for specific cortisol metabolites. The test measures the total amount of these metabolites excreted over the full day, reflecting your body’s cortisol production over time.

What the Results Mean

Higher-than-expected levels may suggest conditions like Cushing’s syndrome, while lower-than-expected levels could indicate adrenal insufficiency or other hormonal imbalances. Your doctor will interpret the results in the context of your symptoms and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Urine Cortisol Metabolite

Frequently asked questions

What are the typical reference ranges for this test?
Reference ranges can vary by lab and may not be widely standardized. Your doctor will provide your specific results and compare them to your unique health needs. These tests are often used to guide treatment decisions rather than provide absolute cutoffs alone.
How long does it take to get my results?
Results are typically available within 1 to 2 weeks after the sample is submitted, depending on lab processing times. Follow-up with your healthcare provider to discuss the findings.
Can I take medications before this test?
Some medications, including certain steroids or blood pressure drugs, may affect cortisol levels. Always inform your doctor or lab technician about all medications you’re taking to avoid interference with the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy