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Cortisol with Corticotropin Stimulation Test (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures your cortisol levels before and after a dose of corticotropin (ACTH) to check how well your body responds to stress hormones.

Quick summary

["Assesses adrenal function and helps diagnose conditions like Cushing’s syndrome or adrenal insufficiency."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94873-7
Short code
Cortis F p corticotropin stim Pnl SerPl
Synonyms
Cortis F p corticotropin stim Pnl SerPl
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect an issue with your adrenal glands, such as overproduction (Cushing’s syndrome) or underproduction (adrenal insufficiency). It’s also used to evaluate how your body reacts to stress hormones like cortisol.

How the Test Works

You’ll first have a blood sample drawn to measure your baseline cortisol level. Then, you’ll receive a synthetic form of ACTH (corticotropin) to stimulate your adrenal glands. Another blood sample is taken shortly after to measure your cortisol response.

What to Expect Before the Test

No special preparation is needed, but avoid taking certain medications (like steroids) that could affect cortisol levels without discussing it with your doctor first.

What to Expect During the Test

You’ll have a few blood draws: one before stimulation and one after. The process is quick and usually done in a clinic or lab.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal results can indicate problems like Cushing’s syndrome (high cortisol), adrenal insufficiency (low cortisol), or pituitary issues affecting ACTH production. Low response to ACTH stimulation may suggest adrenal gland dysfunction.

Short name

Cortisol Stim Test

Frequently asked questions

What does a low cortisol response mean?
A low cortisol response after ACTH stimulation may suggest adrenal insufficiency or failure of the adrenal glands to produce cortisol properly. This could be due to conditions like Addison’s disease or damage to the adrenal glands.
What does a high cortisol response mean?
A high cortisol response may not always indicate a problem, but it can sometimes suggest overactivity of the pituitary gland or other hormonal imbalances. Your doctor will interpret results in the context of your symptoms and other tests.
How often can I get this test?
This test is typically done once, but your doctor may recommend follow-up tests if needed. Frequency depends on your symptoms and medical history.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy