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Cortisol (Mass Concentration) in Serum

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a hormone produced by your adrenal glands, in your blood. Cortisol helps regulate metabolism, immune response, and stress reactions.

Quick summary

["Cortisol helps manage stress and metabolism; abnormal levels may signal health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
11040-3
Short code
Cortis F Ur-mCnc
Synonyms
Cortis F Ur-mCnc
Category
hormone
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your doctor may order cortisol testing to diagnose or monitor conditions like Cushing’s syndrome (too much cortisol), Addison’s disease (too little cortisol), or adrenal gland issues. It can also help evaluate stress responses or rule out hormone imbalances.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if cortisol is measured at specific times (e.g., morning or evening). Bring any medications you’re taking to your appointment.

How the Test Is Performed

A blood sample is drawn from a vein in your arm. The sample is sent to a lab for analysis using a sensitive method to measure cortisol concentration.

What the Results Mean

Results are compared to general reference ranges for cortisol, but values can vary based on timing (e.g., higher in the morning, lower at night). Your doctor will interpret results in the context of your symptoms and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Cortisol levels outside normal ranges can indicate underlying health issues, such as hormonal disorders, stress-related conditions, or adrenal gland problems. Chronic high cortisol may lead to weight gain, fatigue, or skin changes, while low cortisol can cause dizziness, weight loss, or muscle weakness.

Short name

Cortisol Level

Frequently asked questions

What causes cortisol levels to be tested?
Cortisol testing is often done if you have symptoms like unexplained weight changes, fatigue, high blood pressure, or skin problems. It may also be part of a broader evaluation for conditions like Cushing’s syndrome, Addison’s disease, or to assess stress responses.
How long does it take to get the results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Your doctor will contact you once they are ready.
Can I take medications before the test?
Yes, but inform your doctor or lab about all medications, including over-the-counter drugs or supplements, as some may affect cortisol levels. Avoid fasting-related medications unless instructed otherwise.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy