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Cortisol and Cortisone Panel (24-Hour Urine)

Published: August 9, 2026Updated: August 16, 2026

This test measures your body’s natural production of cortisol and cortisone over a full day through your urine. It helps assess adrenal function, especially in conditions like Cushing’s syndrome or adrenal insufficiency.

Quick summary

["This panel helps diagnose hormonal imbalances affecting stress response and metabolism."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
101315-0
Short code
Cortisol + Cortisone panel Ur
Synonyms
Cortisol + Cortisone panel Ur
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
6.2
Ref high
19.4
Critical range
3 N/A – 50 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82533
SNOMED
34350002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend this test if you have symptoms like unexplained weight changes, high blood pressure, fatigue, or signs of hormonal disorders. It’s also used to monitor conditions like Cushing’s syndrome or adrenal gland issues.

What to Expect Before the Test

You’ll collect your urine for 24 hours, starting after you wake up and ending the next morning. Keep a record of the time each urine sample is produced. Drink plenty of fluids to help your body pass all the cortisol naturally produced during the day.

What the Test Measures

The test looks at two hormones: cortisol, which helps regulate stress and metabolism, and cortisone, a form of cortisol that your body converts. Elevated levels may suggest overproduction, while low levels could indicate adrenal problems.

How the Results Are Used

Your doctor will compare your results to what’s expected for a healthy adult. High levels might point to conditions like Cushing’s syndrome, while low levels could signal adrenal insufficiency or other hormonal disorders.

Reference range

Morning: 6.2-19.4 μg/dLN/A

Specimen: serum

Typically collected in the morning (8-10 AM). Inform your provider if taking steroid medications.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Free Cortisol & Cortisone

Frequently asked questions

How is this test different from a simple blood cortisol test?
Unlike blood tests, which measure cortisol at a single point in time, this urine test captures cortisol production over 24 hours, giving a more comprehensive picture of your body’s natural cortisol levels and helping detect chronic imbalances.
What should I do if I miss any urine samples during the 24-hour period?
If you miss any urine, start fresh the next day with a new collection. Do not skip days—this can affect the accuracy of the results. Notify your doctor immediately if this happens.
Can I take medications that might affect cortisol levels before the test?
Yes, some medications (like steroids, birth control pills, or certain antidepressants) can influence cortisol levels. Always inform your doctor or lab technician about all medications you’re taking, including over-the-counter drugs and supplements.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy