Cortisol, Serum (Mass Concentration) – Quantified by Immunoassay
This test measures the amount of cortisol, a natural steroid hormone produced by your adrenal glands. Cortisol helps regulate metabolism, immune response, and stress reactions.
["Cortisol helps manage stress and supports normal body functions; abnormal levels may signal health concerns."]
Key facts at a glance
- LOINC
- 50421-7
- Short code
- Cortis pre 100 ug CRH IV SerPl-mCnc
- Synonyms
- Cortis pre 100 ug CRH IV SerPl-mCnc
- Category
- hormone
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 86701
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your doctor may order cortisol testing to diagnose conditions like Cushing’s syndrome (too much cortisol), Addison’s disease (too little cortisol), or to evaluate adrenal gland function during stress or illness. It can also help assess hormone imbalances or response to treatments.
What to Expect Before the Test
No special preparation is needed, but you may be asked to avoid certain medications (like steroids) or timing your sample around specific events (e.g., morning/evening). If you’re taking medications, check with your provider.
How the Test Is Performed
A blood sample is drawn from a vein in your arm, typically in a clinic or lab. The serum (liquid part of blood) is separated and tested for cortisol levels using an immunoassay method.
What the Results Mean
Results are compared to general reference ranges, but individual ranges can vary. High cortisol may indicate overproduction (e.g., from tumors or chronic stress), while low levels may suggest adrenal insufficiency or other disorders.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal cortisol levels can signal serious conditions, such as hormonal imbalances, adrenal tumors, or chronic stress disorders. For example, persistently high cortisol may cause weight gain, fatigue, or skin changes, while low levels can lead to weakness, dizziness, or darkening of the skin. Your doctor will interpret results in the context of your symptoms and medical history.
Short name
Cortisol Level
Frequently asked questions
What causes cortisol levels to be tested?
How long does it take to get results?
Can I take anything before or after the test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy